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AssigneesAlbert Einstein College of Medicine of Yeshiva University, The Board of Trustees of the Leland Stanford Jr. University, Whitehead Institute for Biomedical Research
InventorsBarry R. Bloom, Ronald W. Davis, William R. Jacobs, Jr., Richard A. Young, Robert N. Husson
Estimating the term…
Recombinant mycobacterial vaccine vehicles capable of expressing DNA of interest which encodes at least one protein antigen for at least one pathogen against which an immune response is desired and which can be incorporated into the mycobacteria or stably integrated into the mycobacterial genome. The vaccine vehicles are useful for administration to mammalian hosts for purposes of immunization. A recombinant vector which replicates in E. coli but not in mycobacteria is also disclosed. The recombinant vector includes 1) a mycobacterial gene or portions thereof, necessary for recombination with homologous sequences in the genome of mycobacteria transformed with the recombinant plasmid; 2) all or a portion of a gene which encodes a polypeptide or protein whose expression is desired in mycobacteria transformed with the recombinant plasmid; 3) DNA sequences necessary for replication and selection in E. coli; and 4) DNA sequences necessary for selection in mycobacteria (e.g., drug resistance). The present invention also relates to two types of recombinant vectors useful in introducing DNA of interest into mycobacteria, where it is expressed. One type of vector is a recombinant phasmid capable of replicating as a plasmid in E. coli and of lysogenizing a mycobacterial host. The other type of vector is a recombinant plasmid which can be introduced into mycobacteria, where it is stably maintained extrachromosomally.
A method of making a vaccine for inducing an immune response in a mammalian host against one or more pathogens, comprising introducing into Mycobacterium bovis-BCG DNA of interest encoding at least one protein antigen for each of said pathogens, wherein the DNA of interest is under control of a promoter other than a mycobacterial heat shock gene promoter or a mycobacterial stress protein gene promoter and the Mycobacterium bovis-BCG expresses the DNA of interest thereby inducing an immune response to the protein antigen in a mammalian host.
A vaccine comprising a nonpathogenic, recombinant mycobacterium having DNA of interest encoding a protein or a polypeptide to which an immune response is desired integrated into genomic DNA of the recombinant mycobacterium, wherein the recombinant mycobacterium expresses the DNA of interest thereby inducing an immune response to the protein or polypeptide in a mammalian host.
A method of inducing an immune response in a mammalian host against one or more pathogens, comprising administering to said host a nonpathogenic, recombinant mycobacterium, said recombinant mycobacterium having integrated into its genome DNA encoding at least one protein antigen for each of said pathogens, wherein the recombinant mycobacterium expresses the DNA thereby inducing an immune response to the protein antigen in a mammalian host.
A vaccine comprising a nonpathogenic, recombinant mycobacterium having incorporated therein heterologous DNA encoding an antigen against which an immune response is to be elicited, wherein the DNA is under control of a promoter other than a mycobacterial heat shock gene promoter or a mycobacterial stress protein gene promoter and the recombinant mycobacterium expresses the heterologous DNA thereby inducing an immune response to the antigen in a mammalian host.
A method of eliciting an immune response in a mammal against one or more antigens, comprising:
A method of making a vaccine for inducing an immune response in a mammalian host against one or more pathogens, comprising introducing into mycobacterium DNA of interest encoding at least one protein antigen for each of said pathogens, wherein the DNA is integrated into genomic DNA of the mycobacterium and the mycobacterium expresses the DNA of interest thereby inducing an immune response to the protein antigen in a mammalian host.
A method of making a vaccine for inducing an immune response in a mammalian host against one or more pathogens, comprising introducing into mycobacterium DNA of interest encoding at least one protein antigen for each of said pathogens, wherein the DNA of interest is under control of a promoter that is not a mycobacterial heat shock gene promoter or a mycobacterial stress protein gene promoter and the mycobacterium expresses the DNA of interest thereby inducing an immune response to the protein antigen in a mammalian host.
A vaccine comprising a nonpathogenic, recombinant mycobacterium having incorporated therein heterologous DNA encoding an antigen against which an immune response is to be elicited, wherein the DNA is under control of a promoter which is not a mycobacterial gene promoter and which expresses the DNA in mycobacteria, and the recombinant mycobacterium expresses the heterologous DNA thereby inducing an immune response to the antigen in a mammalian host.
A method of making a vaccine for inducing an immune response in a mammalian host against one or more pathogens, comprising introducing into mycobacterium DNA of interest encoding at least one protein antigen for each of said pathogens, wherein the DNA is under control of a promoter which is not a mycobacterial gene promoter and which expresses the DNA in mycobacteria, and the mycobacterium expresses the DNA of interest thereby inducing an immune response to the protein antigen in a mammalian host.
Dependencies are inferred from the claim text.