Diagnostic kit for assaying sucrose in physiological fluids

A kit for use in a diagnostic method for detecting sucrose in blood or urine, said kit comprising:

• (a) a first container having therein a first reagent mixture comprising ATP, NAD, hexokinase, G-6-PDH, and a buffer, present in amounts effective to provide a first reagent solution comprising (i) ATP in a concentration in the range from about 0.5 mM to about 5.0 mM, (ii) NAD in a concentration in the range from about 0.5 mM to about 3.0 mM, (iii) hexokinase in a concentration in the range from about 200 U/L to about 2000 U/L, and (iv) G-6-PDH in a concentration in the range from about 500 U/L to about 2500 U/L, the first reagent solution having a pH in the range from about 7 to about 8;
• (b) a second container having therein a second reagent mixture comprising ATP, NAD, hexokinase, G-6-PDH, invertase, and a buffer, present in amounts effective to provide a second reagent solution comprising (i) ATP in a concentration in the range from about 0.0 mM to about 5.0 mM, (ii) NAD in a concentration in the range from about 0.0 mM to about 3.0 mM, (iii) hexokinase in a concentration in the range from about 0 U/L to about 2000 U/L, (iv) G-6-PDH in a concentration in the range from about 0 U/L to about 2500 U/L, and (v) invertase in a concentration in the range from about 0 U/L to about 2500 U/L, and (v) invertase in a concentration in the range from about 50 kU/L to about 500 kU/L, the second reagent solution having a pH in the range from about 7 to about 8;
• and
• (c) a third container having a sucrose solution as a standard and a preservative;
• wherein said kit is stable when stored or transported.