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AssigneeThe Regents of the University of California
InventorsMichael Karin, Joseph A. DiDonato, David M. Rothwarf, Makio Hayakawa, Ebrahim Zandi
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The present invention provides a substantially purified nucleic acid molecule encoding a serine protein kinase (IκB kinase) that phosphorylates a protein (IκB) that inhibits the activity of the NF-κB transcription factor, vectors comprising such a nucleic acid molecule and host cells containing such vectors. In addition, the invention provides nucleotide sequences that can bind to a nucleic acid molecule of the invention, such nucleotide sequences being useful as probes or as antisense molecules. The invention also provides a substantially purified IκB kinase, which is a polypeptide that can phosphorylate an IκB protein, and peptide portions of the IκB kinase. In addition, the invention provides anti-IκB kinase antibodies, which specifically bind to an IκB kinase, and IκB kinase-binding fragments of such antibodies. The invention further provides methods of substantially purifying an IκB kinase, methods of identifying an agent that can alter the association of an IκB kinase with a second protein, and methods of identifying proteins that can interact with an IκB kinase.
An isolated human protein kinase, which phosphorylates serine-32 and serine-36 of IκBα and which has an apparent molecular mass of 84 kiloDaltons as determined by SDS-polyacrylamide gel electrophoresis in an 8% gel under reducing conditions.
An isolated human protein kinase, which phosphorylates serine-32 and serine-36 of IκBα and which has an apparent molecular mass of 84 kiloDaltons as determined by SDS-polyacrylamide gel electrophoresis in an 8% gel under reducing conditions, comprising an amino acid sequence as shown in SEQ ID NO:
A peptide portion of a polypeptide having the amino acid sequence shown in SEQ ID NO:
A peptide portion of a polypeptide having the amino acid sequence shown in SEQ ID NO:
Dependencies are inferred from the claim text.