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US 6,267,858 B1Granted
High throughput screening assay systems in microscale fluidic devices
AssigneeCaliper Technologies Corp.
InventorsJ. Wallace Parce, Anne R. Kopf-Sill, Luc J. Bousse
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Abstract
The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays. In particular, the devices and methods of the invention are useful in screening large numbers of different compounds for their effects on a variety of chemical, and preferably, biochemical systems.
Claims14
A microfluidic device, comprising:
- a body having at least a first microscale channel disposed therein;
- at least a first reservoir in fluid communication with the first microscale channel, the first reservoir having no electrodes disposed therein;
- at least a second reservoir connected to the first reservoir by a second channel;
- and
- at least a first electrode place into contact with a fluid disposed in the second reservoir:
- wherein the second channel is configured to retard the movement of one or more degradable components between the electrode and the first microscale channel, the second channel comprising one or more of:
- a cross-sectional dimension that is smaller than a cross-sectional dimension of the first channel;
- and
- a cross-sectional dimension that is smaller than a cross-sectional dimension of the first channel.
6 more elements
A microfluidic device, comprising:
- a body having at least a first microscale channel disposed therein;
- at least a first reservoir in fluid communication with the first microscale channel, the first reservoir having no electrodes disposed therein;
- at least a second reservoir connected to the first reservoir by a second channel, wherein the second channel comprises a frit disposed therein;
- and
- at least a first electrode place into contact with a fluid disposed in the second reservoir.
2 more elements
A microfluidic device, comprising:
- a body having at least a first microscale channel disposed therein;
- at least a first reservoir in fluid communication with the first microscale channel, the first reservoir having no electrodes disposed therein;
- at least a second reservoir connected to the first reservoir by a second channel;
- and
- at least a first electrode place into contact with a fluid disrosed in the second reservoir;
- wherein a fluid level in the first reservoir is higher than a fluid level in the second reservoir.
3 more elements
A microfluidic device, comprising:
- a body having at least a first channel disposed therein;
- first and second reservoirs in fluid communication with first and second ends of the first channel, respectively;
- first and second electrodes electrically connected to a fluid in the first and second reservoirs, respectively, to provide a voltage gradient through the first channel between the first and second reservoirs;
- and
- wherein the first electrode is separated from the first end of the first channel by a first structure which prevents migration of an electrically degraded chemical species from a surface of the first electrode to the first end of the first channel.
2 more elements
Dependencies are inferred from the claim text.