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US 6,267,579 B1Granted
Apparatus for making a gradient gel
AssigneeClinical Laboratory Development Group, Inc.
InventorsXianzhou Li, Wendy Innis-Whitehouse, Ngoc-Anh Le, Keith Gray
Life of this patent
Estimating the term…
Abstract
An apparatus and method for making a gradient gel. The present invention in one embodiment is a gel-making system that has a reservoir for holding a solution. The reservoir is connected to a movable arm through a tubing. The tubing has two ends: one end is in fluid communication with the reservoir, and the other, an open end, is received by the movable arm. A gel holder having an internal gel chamber is placed underneath the movable arm for receiving the solution. In operation, the movement of the movable arm causes the open end of the tubing to move along with it and the open end of the tubing delivers the solution in motion to the internal gel chamber to form the gel.
Claims58
An apparatus for making a gel, comprising:
- a. a reservoir for holding a solution;
- b. a tubing having a first end and a second end, wherein the first end of the tubing is in fluid communication with the reservoir;
- c. a movable arm for receiving the second end of the tubing;
- and
- d. a gel holder having an internal gel chamber placed underneath the second end of the tubing for receiving the solution therefrom, whereby the movement of the movable arm causes the second end of the tubing to move along with the movable arm and the second end of the tubing delivers the solution in motion to the internal gel chamber to form the gel.
2 more elements
An apparatus for making a gel, comprising:
- a. a solution including at least an acrylamide;
- b. a reservoir for holding the solution;
- c. a tubing having a first end and a second end, wherein the first end of the tubing is in fluid communication with the reservoir;
- d. a movable arm for receiving the second end of the tubing;
- e. a gel holder having an internal gel chamber placed underneath the second end of the tubing for receiving the solution therefrom, whereby the movement of the movable arm causes the second end of the tubing to move along with the movable arm and the second end of the tubing delivers the solution in motion to the internal gel chamber to form the gel.
2 more elements
An apparatus for making a gradient gel, comprising:
- a. a reservoir having a first container and a second container, wherein the first container holds a first solution and the second container holds a second solution;
- b. a channel connecting the first container and the second container;
- c. an outlet connected to the second container communicating with the channel so that a fluid of the first solution and the second solution is formed at the outlet;
- d. a tubing having a first end and a second end, wherein the first end of the tubing is in fluid communication with the outlet;
- e. a movable arm for receiving the second end of the tubing;
- and
- f. a gel holder with arm internal gel chamber placed underneath the second end of the tubing for receiving the fluid, wherein the chamber has a longitudinal axis,
- whereby the movement of the movable arm along the longitudinal axis causes the second end of the tubing to move along with the movable arm and the second end of the tubing delivers the fluid in motion in the gel chamber to form the gradient gel.
5 more elements
An apparatus for making a gradient gel, comprising:
- a. a first solution which includes a polyacrylamide solution selected from the group consisting of medium concentration polyacrylamide solution and low concentration polyacrylamide solution, and a second solution;
- b. a reservoir having a first container and a second container, wherein the first container holds the first solution and the second container holds the second solution;
- c. a channel connecting the first container and the second container;
- d. an outlet connected to the second container communicating with the channel so that a fluid of the first solution and the second solution is formed at the outlet;
- e. a tubing having a first end and a second end, wherein the first end of the tubing is in fluid communication with the outlet;
- f. a movable arm for receiving the second end of the tubing;
- and
- g. a gel holder with an internal gel chamber placed underneath the second end of the tubing for receiving the fluid, wherein the chamber has a longitudinal axis,
- whereby the movement of the movable arm along the longitudinal axis causes the second end of the tubing to move along with the movable arm and the second end of the tubing delivers the fluid in motion in the gel chamber to form the gradient gel.
6 more elements
An apparatus for making a gradient gel, comprising:
- a. a first solution and a second solution which includes a polyacrylamide solution selected from the group consisting of high concentration polyacrylamide solution and medium polyacrylamide solution;
- b. a reservoir having a first container and a second container, wherein the first container holds the first solution and the second container holds the second solution;
- c. a channel connecting the first container and the second container;
- d. an outlet connected to the second container communicating with the channel so that a fluid of the first solution and the second solution is formed at the outlet;
- e. a tubing having a first end and a second end, wherein the first end of the tubing is in fluid communication with the outlet;
- f. a movable arm for receiving the second end of the tubing;
- and
- g. a gel holder with an internal gel chamber placed underneath the second end of the tubing for receiving the fluid, wherein the chamber has a longitudinal axis,
- whereby the movement of the movable arm along the longitudinal axis causes the second end of the tubing to move along with the movable arm and the second end of the tubing delivers the fluid in motion in the gel chamber to form the gradient gel.
6 more elements
An apparatus for mailing a gradient gel, comprising:
- a. a plurality of containers holding a plurality of solutions, wherein each container holds one solution and communicates with at least one neighboring container;
- b. an outlet communicating with at least one of the plurality of the containers so that a fluid of at least two solutions from the plurality of solutions is formed at the outlet;
- c. a tubing having a first end and a second end, wherein the first end of the tubing is in fluid communication with the outlet;
- d. a movable arm for receiving the second end of the tubing;
- and
- e. a gel holder with an internal gel chamber placed underneath the second end of the tubing for receiving the fluid, wherein the chamber has a longitudinal axis,
- whereby the movement of the movable arm along the longitudinal axis causes the second end of the tubing to move along with the movable arm and the second end of the tubing delivers the fluid in motion in the gel chamber to form the gradient gel.
4 more elements
An apparatus for making a gradient gel, comprising:
- a. means for holding a plurality of solutions;
- b. means for communicating with the holding means so that a fluid with at least two solutions from the plurality of the solutions is formed therein;
- c. means for transferring the fluid;
- d. means for moving the transferring means;
- and
- e. means located underneath the transferring means for receiving the fluid, wherein the receiving means has a longitudinal axis,
- whereby the movement of the moving means along the longitudinal axis causes the transferring means to move along with the moving means and the transferring means deliver the fluid in motion in the receiving means to form a gradient gel.
4 more elements
An apparatus for making a gradient gel, comprising:
- a. a horizontal base with a plurality of corners;
- b. a plurality of supporting posts bearing against the plurality of the corners;
- c. a plurality of horizontal bars connecting the plurality of the supporting posts to form a housing having a first longitudinal axis, wherein the plurality of the horizontal bars are movably connected to the plurality of the supporting posts so that the heights of the plurality of the horizontal bars relative to the horizontal base are adjustable individually, and at least one of the plurality of horizontal bars is perpendicular to the first longitudinal axis;
- d. a supporting board bearing against the base and perpendicular to the first longitudinal axis;
- e. a shaft connecting the parallel horizontal bar perpendicular to the first longitudinal axis and the supporting board so as to divide the housing into a first room and a second room, wherein the shaft further comprises:
- i. an elongated body;
- ii. an external thread on the elongated body;
- and
- iii. a second longitudinal axis, thereby the shaft is rotatable around the second longitudinal axis;
- f. a motor operatively connected to the shaft;
- g. a reservoir located above the housing having a first container and a second container, wherein the first container holds a first solution and the second container holds a second solution;
- h. a channel connecting the first container and the second container;
- i. an outlet connected to the second container communicating with the channel so that a fluid of the first solution and the second solution is formed at the outlet;
- j. a tubing having a first end and a second end, wherein the first end of the tubing is in fluid communication with the outlet;
- k. a movable arm for receiving the second end of the tubing, wherein the movable arm comprises:
- i. an internally threaded bore adapted to mate with the shaft through the external thread on the elongated body of the shaft;
- and
- ii. at least two openings, each sized to allow the second end of the tubing to pass through and hold at least a portion of the tubing proximate to the second end of the tubing;
- l. a first switch mounted on the supporting board and a second switch mounted on the at least one of the plurality of horizontal bars, wherein each of the switches changes the direction of rotation of the shaft around the second longitudinal axis when it is activated by the movable arm;
- and
- m. a gel holder with an internal gel chamber placed underneath the movable arm bearing against the base in one of the first room or the second room for receiving the fluid delivered through the second end of the tubing in motion to form the gradient gel.
18 more elements
An apparatus for making a plurality of gradient gels, comprising:
- a. a plurality of reservoirs, each having:
- i. a first container and a second container, wherein the first container holds a first solution and the second container holds a second solution;
- ii. a channel connecting the first container and the second container;
- and
- iii. an outlet connected to the second container communicating with the channel so that a fluid of the first solution and the second solution is formed at the outlet, thereby a plurality of fluids are formed at the plurality of the outlets of the plurality of the reservoirs;
- b. a plurality of tubing, each having a first end and a second end and each communicating with one of the plurality of reservoirs through the connection of the second end of the tubing and the outlet of that reservoir;
- c. a movable arm for receiving a plurality of the second ends of the plurality of the tubing;
- and
- d. a plurality of gel holders, each having an internal gel chamber being placed underneath the movable arm for receiving the fluid from one of the plurality of the second ends, wherein the plurality of the gel holders are positioned in parallel thereby defining a longitudinal axis,
- whereby the movement of the movable arm moves along the longitudinal axis causes the plurality of the second ends of the plurality of the tubings to move along with the movable arm and each second end of the plurality of the tubings delivers one fluid of the plurality of the fluids in motion into one gel chamber to form one of the plurality of the gradient gels.
7 more elements
Dependencies are inferred from the claim text.