Method for measuring changes in osmotic pressure

A method for monitoring changes in osmotic pressure of a sample as a function of time comprising the steps of:

• (a) placing a sample of a solution at an initial temperature into a sample cell above a membrane supported in the sample cell;
• (b) sealing the sample in the sample cell;
• (c) introducing gas at an elevated pressure to the sample cell to generate a dialyzate by allowing a portion of the solution to dialyze through the membrane and enter a transparent dialyzate exit tube connected to the sample cell;
• (d) varying the elevated pressure of the gas until equilibrium across the membrane is substantially achieved;
• (e) indicating osmotic equilibrium across the membrane with a substantially stationary dialyzate meniscus in the transparent dialyzate exit tube;
• (f) changing the temperature of the solution over time;
• (g) measuring the osmotic pressure at predetermined intervals of time;
• and
• (h) calculating the changes in the osmotic pressure.

A method for monitoring changes in osmotic pressure of a sample as a function of time comprising the steps of:

• (a) placing a sample of a solution at an initial temperature into a sample cell above a membrane supported in the sample cell;
• (b) adding reactants to the sample;
• (c) sealing the sample and reactants in the sample cell;
• (d) introducing gas at an elevated pressure to the sample cell to generate a dialyzate by allowing a portion of the solution to dialyze through the membrane;
• (e) varying the elevated pressure of the gas until equilibrium across the membrane is substantially achieved, equilibrium being indicated by a substantially stationary dialyzate meniscus in a transparent dialyzate exit tube connected to the sample cell;
• (f) measuring the osmotic pressure at predetermined intervals of time;
• and
• (g) calculating the changes in the osmotic pressure.

A method for monitoring changes in osmotic pressure of a sample as a function of time comprising the steps of:

• (a) placing a sample of a solution at an initial temperature into a sample cell above a membrane supported in the sample cell;
• (b) adding reactants to the sample;
• (c) sealing the sample and reactants in the sample cell;
• (d) introducing gas at an elevated pressure to the sample cell to generate a dialyzate by allowing a portion of the solution to dialyze through the membrane and enter a transparent dialyzate exit tube open to atmosphere and connected to sample cell;
• (e) varying the elevated pressure of the gas to obtain a substantially stationary dialyzate meniscus visually observable in the transparent dialyzate exit tube, the substantially stationary dialyzate meniscus indicating osmotic equilibrium across the membrane;
• (f) measuring the osmotic pressure at predetermined intervals of time;
• and
• (g) calculating the changes in the osmotic pressure.