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AssigneeThe Salk Institute for Biological Studies
InventorsRonald M. Evans, Michael B. Mc Keown, Anthony E. Oro, William A. Segraves, Tso-Pang Yao
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In accordance with the present invention, it has been discovered that various members of the steroid/thyroid superfamily of receptors can interact with the insect-derived ultraspiracle receptor, to form multimeric species. Accordingly, the interaction of at least one member of the steroid/thyroid superfamily of receptors with at least the dimerization domain of the ultraspiracle receptor modulates the ability of said member of the steroid/thyroid superfamily of receptors to trans-activate transcription of genes maintained under hormone expression control in the presence of the cognate ligand for said member of the superfamily.
A method to indirectly induce or repress, in an in vitro expression system or in cells in culture, the transcription activation of a first gene by a member of the steroid/thyroid hormone superfamily of receptors which associates with at least the dimerization domain of ultraspiracle receptor, in the presence of ligand for said member, wherein the expression of said gene is maintained under the control of a hormone response element to which said member binds, said method comprising:
A method to directly or indirectly induce or repress, in an expression system, the transcription activation of a gene by a member of the steroid/thyroid superfamily of receptors which associates with at least the dimerization domain of ultraspiracle receptor, in the presence of ligand for said member, and in the further presence of at least the dimerization domain of an ultraspiracle receptor, wherein the expression of said gene is maintained under the control of a hormone response element to which said member is capable of binding, said method comprising:
A method according to claim 4 wherein said compound which prevents association of said member with said ultraspiracle receptor is an anti-ultraspiracle antibody.
A method for the expression of a recombinant product detrimental to a host organism, said method comprising:
Dependencies are inferred from the claim text.