Compositions, kits, and methods for modulating survival and differentiation of multi-potential hematopoietic progenitor cells
AssigneeThe Board of Trustees of Northwestern University
InventorsIsaac Cohen, Phil Lefebvre, Jiandie Lin, Daniel Linzer
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Abstract
The invention includes compositions, kits, and methods for modulating survival and differentiation of mammalian multi-potential hematopoietic progenitor cells using a placental glycoprotein hormone of the murine prolactin family, namely either murine prolactin-like protein E or murine prolactin-like protein F. The compositions, kits, and methods described herein can be used, for example, for in vitro or ex vivo expansion of hematopoietic precursor cells or to treat a disorder associated with aberrant hematopoiesis (e.g., pre-eclampsia and thrombocytopenia).
Claims20
A method of maintaining mammalian hematopoietic progenitor cells in vitro, the method comprising maintaining the cells in vitro in a nutritive medium comprising murine prolactin-like protein E (mPLP-E).
A method of inducing proliferation of mammalian hematopoietic progenitor cells in vitro, the method comprising maintaining the cells in vitro in a nutritive medium comprising mPLP-E.
A method of expanding mammalian hematopoietic progenitor cells in vitro, the method comprising maintaining the cells in vitro in a nutritive medium comprising mPLP-E.
A method of inducing differentiation of mammalian hematopoietic progenitor cells in vitro, the method comprising maintaining the cells in vitro in a nutritive medium comprising mPLP-E.
A method of inducing mammalian hematopoietic progenitor cells to differentiate in vitro into megakaryocytes, the method comprising maintaining the cells in vitro in a nutritive medium comprising mPLP-E.
A method of inducing mammalian megakaryocytes to differentiate into platelets, the method comprising contacting the megakaryocytes with mPLP-E.
In an in vitro method of maintaining mammalian hematopoietic progenitor cells, the improvement comprising
- maintaining the cells in the presence of mPLP-E.
Dependencies are inferred from the claim text.