Method of cloning porcine animals
AssigneeInfigen, Inc.
InventorsPhilip Damiani, Jeffrey M. Betthauser, Erik J. Forsberg, Michael D. Bishop
Life of this patent
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Abstract
The present invention relates to materials and methods for cloning porcine animals. The invention relates in part to totipotent cells useful for cloning porcine animals, porcine embryos produced from such cells by employing nuclear transfer techniques, and porcine animals that arise from such cells and embryos.
Claims47
A method for preparing a porcine embryo capable of developing into a live-born porcine animal, the method comprising:
- (a) obtaining a totipotent porcine nuclear donor cell from a cell culture;
- (b) forming a cybrid by translocating said totipotent porcine nuclear donor cell, or nucleus thereof, into an enucleated porcine oocyte, wherein prior to enucleation, said oocyte has been matured for between 42 and 56 hours, and before, during or after said translocation, activating said oocyte;
- (c) culturing said cybrid to establish said porcine embryo.
A process for preparing a porcine animal, the method comprising:
- (a) obtaining a totipotent porcine nuclear donor cell from a cell culture;
- (b) forming a cybrid by translocating said totipotent porcine nuclear donor cell, or nucleus thereof, into an enucleated porcine oocyte, wherein prior to enucleation, said oocyte has been matured for between 42 and 56 hours, and before, during or after said translocation, activating said oocyte;
- (c) culturing said cybrid to establish said porcine embryo;
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- (d) transferring said porcine embryo into a recipient porcine female so as to produce a fetus that undergoes full fetal development and parturition to generate a live-born porcine animal.
A process for preparing a totipotent porcine fetus, the method comprising:
- (a) obtaining a totipotent porcine nuclear donor cell from a cell culture;
- (b) forming a cybrid by translocating said totipotent porcine nuclear donor cell, or nucleus thereof, into an enucleated porcine oocyte, wherein prior to enucleation, said oocyte has been matured for between 42 and 56 hours, and before, during or after said translocation, activating said oocyte;
- (c) culturing said cybrid to establish said porcine embryo;
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- (d) transferring said porcine embryo into a recipient porcine female so as to produce a totipotent porcine fetus.
A process for preparing a porcine animal, the method comprising:
- (a) obtaining a porcine nuclear donor cell from a cell culture, wherein said cell is a fetal cell;
- (b) forming a cybrid by nuclear transfer of said totipotent porcine nuclear donor cell, or the nucleus thereof, into an enucleated oocyte, wherein said nuclear transfer comprises:
- (i) placing said porcine nuclear donor cell into the perivitelline space of an enucleated porcine oocyte;
- (ii) fusing said porcine nuclear donor cell and said enucleated porcine oocyte to form a fused cell, wherein, prior to enucleation, said oocyte has been matured for between 42 and 56 hours;
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- (iii) activating said fused cell to form said cybrid;
- (c) culturing said cybrid to establish a porcine embryo;
- and
- (d) transferring said porcine embryo into a recipient female so as to produce a fetus that undergoes full fetal development and parturition to generate said live-born porcine animal.
Dependencies are inferred from the claim text.