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US 6,258,597 B1Granted
Stimulation of hematopoietic cells in vitro
AssigneePoint Therapeutics, Inc.
InventorsWilliam Bachovchin, Barbara Wallner
Life of this patent
Estimating the term…
Abstract
Methods, compositions, and devices for stimulating the number and/or differentiation of hematopoietic cells in vitro are provided. The methods involve contacting the hematopoietic cells with an inhibitor of dipeptidyl peptidase (DPIV) in the absence of exogenously provided cytokines.
Claims20
A method for stimulating hematopoietic cells in vitro comprising:
- (1) contacting the hematopoietic cells with a sufficient amount of an inhibitor of a dipeptidylpeptidase type IV to increase the number of said hematopoietic cells and/or the differentiation state of said hematopoietic cells relative to the number and differentiation of hematopoietic cells that are present in a control culture that is not contacted with the inhibitor but is otherwise subjected to the same culture conditions as the hematopoietic cells which are cultured in the presence of the inhibitor;
- and
- (2) culturing the hematopoietic cells in the presence of the inhibitor and in the absence of exogenous cytokine under conditions and for a time sufficient to increase the number of hematopoietic cells and/or the differentiation of the hematopoietic cells relative to the number of hematopoietic cells that were present in the control culture,
- wherein the hematopoietic cells are selected from the group consisting of umbilical cord blood cells and bone marrow cells.
1 more element
A method for stimulating hematopoietic cells in vitro comprising:
- (1) contacting the hematopoietic cells with a sufficient amount of an inhibitor of a dipeptidylpeptidase type IV to increase the number of said hematopoietic cells and/or the differentiation state of said hematopoietic cells relative to the number and differentiation of hematopoietic cells that are present in a control culture that is not contacted with the inhibitor but is otherwise subjected to the same culture conditions as the hematopoietic cells which are cultured in the presence of the inhibitor;
- and
- (2) culturing the hematopoietic cells in the presence of the inhibitor and in the absence of exogenous cytokine under conditions and for a time sufficient to increase the number of hematopoietic cells and/or the differentiation of the hematopoietic cells relative to the number of hematopoietic cells that were present in the control culture,
- wherein the hematopoietic cells are bone marrow cells.
1 more element
A method for stimulating hematopoietic cells in vitro comprising:
- (1) contacting the hematopoietic cells with a sufficient amount of an inhibitor of a dipeptidylpeptidase type IV to increase the number of said hematopoietic cells and/or the differentiation state of said hematopoietic cells relative to the number and differentiation of hematopoietic cells that are present in a control culture that is not contacted with the inhibitor but is otherwise subjected to the same culture conditions as the hematopoietic cells which are cultured in the presence of the inhibitor;
- and
- (2) culturing the hematopoietic cells in the presence of the inhibitor and in the absence of exogenous cytokine under conditions and for a time sufficient to increase the number of hematopoietic cells and/or the differentiation of the hematopoietic cells relative to the number of hematopoietic cells that were present in the control culture,
- wherein the hematopoietic cells are umbilical cord blood cells.
1 more element
Dependencies are inferred from the claim text.