Method for producing metabolites biologically synthesized via phosphoribosyl pyrophosphate
AssigneeKyowa Hakko Kogyo Co., Ltd.
InventorsMasato Ikeda, Kazuyuki Okamoto, Tetsuo Nakano, Nozomu Kamada
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Abstract
An industrially more advantageous method for the production of metabolites biologically synthesized via phosphoribosyl pyrophosphate (PRPP) is provided, making use of metabolically modified strains in which transketolase activity is deficient or reduced in comparison with the parent strain.
Claims8
A method for increasing productivity of metabolites biologically synthesized via phosphoribosyl pyrophosphate on metabolic pathway, which comprises the steps of:
- selecting a parent strain of microorganism belonging to the genus Corynebacterium, Brevibacterium or Escherichia having transkelolase activity;
- reducing or inactivating said transketolase activity in comparison to that of said parent strain;
- and
- detecting increase in productivity of phosphoribosyl pyrophosphate in said microorganism in comparison to that of said parent strain.
A method for producing metabolites, which comprises the steps of:
- culturing in a medium a microorganism belonging to the genus Corynebacterium, Brevibacterium or Escherichia said microorganism having transketolase activity which is deficient or reduced in comparison with a parent strain thereof;
- forming and accumulating in the culture at least one metabolite including phosphoribosyl pyrophosphate biologically synthesized on the metabolic pathway via phosphoribosyl pyrophosphate;
- and
- recovering said metabolites therefrom.
An isolated microorganism belonging to the genus Corynebacterium, in which transketolase activity is deficient or reduced in comparison with a parent strain thereof, wherein said microorganism is selected from the group consisting of Corynebacterium glutamicum TKT6, Corynebacterium glutamicum TKT6/pPH8 and Corynebacterium glutamicum TKT6/pFM4 1.
Dependencies are inferred from the claim text.