Non-invasive prenatal diagnosis
AssigneeIsis Innovation Limited
InventorsYuk-Ming Dennis Lo, James Stephen Wainscoat
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Abstract
The invention relates to a detection method performed on a maternal serum or plasma sample from a pregnant female, which method comprises detecting the presence of a nucleic acid of foetal origin in the sample. The invention enables non-invasive prenatal diagnosis including for example sex determination, blood typing and other genotyping, and detection of pre-eclampsia in the mother.
Claims27
A method for detecting a paternally inherited nucleic acid of fetal origin performed on a maternal serum or plasma sample from a pregnant female, which method comprises
- amplifying a paternally inherited nucleic acid from the serum or plasma sample and
- detecting the presence of a paternally inherited nucleic acid of fetal origin in the sample.
A method for detecting a paternally inherited nucleic acid on a maternal blood sample, which method comprises:
- removing all or substantially all nucleated and anucleated cell populations from the blood sample,
- amplifying a paternally inherited nucleic acid from the remaining fluid and subjecting the amplified nucleic acid to a test for the Paternally inherited fetal nucleic acid.
A method for performing a prenatal diagnosis on a maternal blood sample, which method comprises
- obtaining a non-cellular fraction of the blood sample
- amplifying a paternally inherited nucleic acid from the non-cellular fraction
- and performing nucleic acid analysis on the amplified nucleic acid to detect paternally inherited fetal nucleic acid.
Dependencies are inferred from the claim text.