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US 6,255,462 B1Granted
Peptide tag for immunodetection and immunopurification
AssigneeUniversity of Iowa Research Foundation
InventorCharles F. Grose
Life of this patent
Estimating the term…
Abstract
This invention discloses the incorporation of a peptide QRQYGDVFKGD (SEQ ID NO:1) from glycoprotein gE of Varicella zoster virus into a protein or polypeptide sequence for immunoisolation, immunopurification and immunodetection.
Claims24
A method for isolating a protein comprising the steps of:
- obtaining a solution comprising a chimeric protein, the chimeric protein comprising a protein linked to one or more heterologous peptide tags, wherein said peptide tag or tags, comprises the amino acid sequence of SEQ ID NO:1, and wherein the chimeric protein binds to antibody to the peptide tag;
- contacting the solution with antibody to the peptide tag;
- and
- isolating the chimeric protein from the solution.
1 more element
A method for identifying a chimeric protein comprising the steps of:
- obtaining a vector that directs expression of a chimeric protein, the protein comprising a first nucleic acid sequence encoding a protein and a second nucleic acid sequence encoding a heterologous peptide tag or tags comprising SEQ ID NO:1 in frame;
- expressing the chimeric protein in a cell;
- preparing a solution of the chimeric protein produced by the cell;
- and
- identifying the chimeric protein by detecting the presence of the peptide tag in the protein.
2 more elements
A kit to identify a protein comprising:
- the nucleic acid sequence SEQ ID NO:1;
- and
- antibody specifically recognizing the peptide encoded by SEQ ID NO:1.
Dependencies are inferred from the claim text.