US 6,255,071 B1 · Mammalian viral vectors and their uses

Abstract

The present invention relates to methods and compositions for the elucidation of mammalian gene function. Specifically, the present invention relates to methods and compositions for improved mammalian complementation screening, functional inactivation of specific essential or non-essential mammalian genes, and identification of mammalian genes which are modulated in response to specific stimuli. In particular, the compositions of the present invention include, but are not limited to, replication-deficient retroviral vectors, libraries comprising such vectors, retroviral particles produced by such vectors in conjunction with retroviral packaging cell lines, integrated provirus sequences derived from the retroviral particles of the invention and circularized provirus sequences which have been excised from the integrated provirus sequences of the invention. The compositions of the present invention further include novel retroviral packaging cell lines.

Claims58

Dependent claims

A method for expressing a heterologous nucleic acid sequence in a mammalian cell, comprising introducing, into chromosomal DNA of mammalian cells isolated in culture, a retroviral vector comprising:

(i) a 3′ LTR sequence for integration of the vector into chromosomal DNA of the mammalian cell;
(ii) a heterologous nucleic acid sequence to be expressed in the mammalian cell;
(iii) proviral excision elements, contained within the 3′ LTR sequence, for excising a proviral form of the vector from chromosomal DNA.

Dependencies are inferred from the claim text.

Facts

Application

08/820,931Filed 19 Mar 1997 · continuation in part

Priority

20 Sep 1996

Granted

3 Jul 2001

Term ends

Not estimatedThe source records don’t support an estimate.

Classification15 codes

C40B 40/02
Libraries per se, e.g. arrays, mixtures: libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained…
C07K 14/005
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from viruses
C12N 15/1037
Isolating an individual clone by screening libraries: screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli…
C12N 15/86
For animal cells: viral vectors

Mammalian viral vectors and their uses

Life of this patent

Mammalian viral vectors and their uses

Life of this patent

Abstract

Claims58