Water-wettable chromatographic media for solid phase extraction
AssigneeWaters Corporation
InventorsEdouard S. P. Bouvier, Randy E. Meirowitz, Patrick D. McDonald
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Abstract
A method for removing an organic solute from a solution, comprising contacting the solution with a polymer formed by copolymerizing one or more hydrophobic monomers and one or more hydrophilic monomers, whereby the solute is adsorbed onto the polymer. The solution can comprise a polar solvent such as a polar organic solvent or water or an aqueous buffer. The hydrophobic monomer can be, for example, divinylbenzene. The hydrophilic monomer can be, for example, a heterocyclic monomer, such as a vinylpyridine or N-vinylpyrrolidone.
Claims32
A method for removing on e or more solutes from a solution, comprising contacting the solution with a water-wettable polymer formed by copolymerizing at least one hydrophilic monomer and at least one hydrophobic monomer having a hydrophobic to hydrophilic monomer ratio sufficient for the polymer to be water-wettable while being effective to retain organic solutes over a range of chromatographic polarities and wherein the polymer comprises greater than at least 12 mole percent of hydrophilic monomer, whereby the solute is adsorbed onto the polymer.
A method for forming a solution of a solute which is suitable for quantitative analysis, comprising the steps of:
- (a) contacting a first solution which includes the solute with a water-wettable polymer formed by copolymerizing at least one hydrophobic monomer and at least one hydrophilic monomer having a hydrophilic to hydrophobic monomer ratio sufficient for the polymer to be water-wettable while being effective to retain organic solutes over a range of chromatographic polarities and wherein the polymer comprises greater than at least 12 mole percent of hydrophilic monomer, whereby the solute is adsorbed onto the resin;
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- (b) washing the sorbent resin with a solvent or a mixture of solvents, thereby desorbing the solute from the polymer and forming a second solution of the solute, said second solution being suitable for quantitative analysis.
A method for forming a solution of a polar organic compound which is suitable for quantitative analysis, comprising the step of contacting an aqueous solution which includes the polar organic solute and one or more additional solutes, the additional solutes being less polar than the polar organic solute, with a water-wettable polymer formed by copolymerizing at least one hydrophobic monomer and at least one hydrophilic monomer having a hydrophilic to hydrophobic monomer ratio sufficient for the polymer to be water-wettable while being effective to retain organic solutes over a range of chromatographic polarities and wherein the polymer comprises greater than at least 12 mole percent of hydrophilic monomer, whereby the additional solutes are adsorbed onto the polymer and the polar organic solute remains in solution.
Dependencies are inferred from the claim text.