Compositions and methods of making embryonic stem cells
AssigneeVanderbilt University
InventorsBrigid L. M. Hogan, Guang-Quan Zhao
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Abstract
The invention relates to cell proliferation, cell differentiation, male infertility, male fertility and to compositions and methods involved therein. Also methods of culturing spermatogonial stem cells with bone morphogenetic protein 8 are disclosed.
Claims9
A method of proliferating mammalian spermatogonial stem cells, consisting of culturing spermatogonial stem cells with a growth factor wherein said growth factor is bone morphogenetic protein 8 to effect proliferation of said cells, wherein said fragment adequately supports survival of spermatocytes in culture.
A method of extending viability of a mammalian spermatogonial cell population, consisting of culturing said spermatogonial cell population with a growth factor wherein said growth factor is bone morphogenetic protein 8 wherein said fragment adequately supports survival of spermatocytes in culture, thereby extending the viability of said cultured spermatogonial cell population.
A method of selectively obtaining a proliferating population of spermatogonial stem cells in culture, consisting of adding a growth factor wherein said growth factor is bone morphogenetic protein 8 to said population of cells, wherein said fragment adequately supports survival of spermatocytes in culture, thereby selectively obtaining a proliferating population of spermatogonial stem cells.
Dependencies are inferred from the claim text.