Seamless capsule for synthesizing biopolymer and method for producing the same
AssigneeMorishita Jintan Co., Ltd.
InventorsItaru Urabe, Tetsuya Yomo, Keizo Yamamoto, Hideki Sunohara, Ryosei Kamaguchi, Yumi Hatano
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Abstract
This invention provides a seamless capsule for synthesizing biopolymer, comprising: (a) an aqueous mixture for synthesizing biopolymer, (b) a seamless capsule layer encapsulating said aqueous mixture, formed from polysaccharide or protein, and, (c) a viscous liquid intermediate layer, present between said aqueous mixture and said seamless capsule layer, which is immiscible with water, wherein said seamless capsule has a diameter of 0.01 to 10.0 mm. This invention can also provide a method for producing the seamless capsule.
Claims23
A method for producing a reaction capsule comprising:
- concurrently extruding a plurality of concentric layers from a plurality of nozzles into a cooling solution, wherein an inner nozzle extrudes an aqueous reaction mixture comprising components required for synthesizing a polynucleotide or a polypeplide;
- an intermediate nozzle extrudes a viscous fluid that is not miscible with water;
- and an outer nozzle extrudes an encapsulating polymer layer formed of polysaccharide, protein, or a mixture thereof to form a reaction capsule;
- and
- isolating the reaction capsule from the cooling solution, wherein the flow rate from the nozzles is controlled to produce a capsule having an approximate diameter of 0.1 to 10.0 mm.
A reaction capsule produced by a process comprising:
- concurrently extruding, a plurality of concentric layers from a plurality of nozzles into a cooling solution, wherein an inner nozzle extrudes an aqueous reaction mixture comprising components required for synthesizing a polynucleotide or a polypeptide;
- an intermediate nozzle extrudes a viscous fluid that is not miscible with water;
- and an outer nozzle extrudes an encapsulating polymer layer formed of polysaccharide, protein, or a mixture thereof;
- and
- isolating from the cooling solution a reaction capsule, wherein the flow rate from the nozzles is controlled to produce a capsule having an approximate diameter of 0.1 to 10.0 mm.
A reaction capsule for producing a nucleic acid or amino acid polymer therein, comprising:
- a core comprising an aqueous reaction mixture for producing a nucleic acid or amino acid polymer;
- a polymer layer encapsulating the core, the polymer layer formed of polysaccharide, protein, or a mixture thereof;
- and
- an intermediate layer comprising a viscous fluid disposed between the core and the encapsulating polymer layer,
- wherein said capsule has a diameter of about 0.01 to about 10.0 mm.
Dependencies are inferred from the claim text.