Method for inactivating non-enveloped viral contaminants with a photosensitizer by increasing viral permeability to the photosensitizer
AssigneeBaxter International Inc.
InventorsSamuel O. Sowemimo-Coker, Raymond P. Goodrich, Jr.
Life of this patent
Estimating the term…
Abstract
A method is presented for inactivating non-enveloped viruses that may be contaminating a biological solution or suspension by mixing the solution or suspension with a photosensitizer to form a mixture, adjusting the operating conditions of the mixture so as to increase the permeability of the viruses to the photosensitizer, and then irradiating the adjusted mixture.
Claims5
A method of inactivating non-lipid-enveloped viral contamination in a biological solution or suspension said solution or suspension, comprising a desired physiologically active component, comprising the steps of:
- a. mixing said biological solution or suspension with a photosensitizer that binds to said non-lipid-enveloped viral contaminant to form a mixture, wherein said photosensitizer has the formula:
- wherein μ is an integer from 1 to 6;
- X is an anionic counterion;
- R1, R2, R3, R4, R5, and R6 are independently a halogen;
- H;
- linear or branched alkyl of 1-10 carbon atoms;
- linear or branched alkoxy of 1-10 carbon atoms;
- —(CH2)m—O—(CH2)p—Z⊕R′R″R′″ or —O—(CH2)n—Z⊕R′R″R′″ wherin n, m and p are independently integers from 1 to 10, Z is N or P, and R′, R″ and R′″ are independently H or linear or branched alkyl of 1 to 10 carbon atoms with the proviso that on each Z atom, not more than two of R′, R″ and R′″ may be H;
- and at least one of R1, R2, R3, R4, R5, or R6 is —(CH2)m—O—(CH2)p—Z⊕R′R″R′″ or —O—(CH2)n—Z⊕R′R″R′″, or a halogen;
- and wherein said photosensitizer inactivates said non-lipid-enveloped viral contaminant upon absorption of radiation;
- b. adjusting an operating condition of said mixture from step (a) in order to increase the permeability of said non-lipid-enveloped viral contaminants to said photosensitizer;
- and
- c. irradiating said mixture from step (b) with ultraviolet, visible or ionizing radiation under conditions whereby substantially all of said non-lipid-enveloped viral contaminants are inactvated and the physiological activity of said component is substantially unimpaired.
A method of inactivating human non-lipid-enveloped viral contaminants in a biological solution or suspension, said solution or suspension comprising a desired physiologically active component, comprising the steps of:
- a. mixing said biological solution or suspension with a photosensitizer that binds to said non-lipid-enveloped viral contaminant to form a mixture, wherein said photosensitizer is selected from the group consisting of photosensitizers having a psoralen or coumarin backbone;
- an intercalator comprising at least one non-H-bonding ionic moiety, at least one halogen atom, or both;
- porphyrins;
- phthalocyanines, quinones, hypericin;
- merocyanine dyes, eosin dyes;
- cinnamic acid;
- caffeic acid;
- ferulic acid;
- gallic acid;
- polyacetylenes;
- thiophenes, and α-terthienyl;
- and wherein said photosensitizer inactivates said non-lipid-enveloped virus upon absorption of radiation;
- b. adjusting an operating condition of said mixture from step (a), wherein said adjustment is selected from the group consisting of adding solvent/detergent and increasing the temperature;
- and
- c. irradiating the mixture from step (b) with ultraviolet, visible or ionizing radiation under conditions whereby substantially all of said non-enveloped virus is inactivated and the physiological activity of said component is substantially unimpaired.
Dependencies are inferred from the claim text.