Assay method utilizing induced luminescence

A composition comprising:

• a) first suspendible particles comprising a chemiluminescent compound that is capable of reacting with singlet oxygen, wherein said first particles have a first specific binding pair member bound to them, and
• b) second suspendible particles having a second specific binding pair member bound to them,
• wherein said second suspendible particles comprise a photosensitizer that is capable, in its excited state, of activating oxygen to its singlet state.

A kit comprising:

• (a) a first composition comprising a member of a specific binding pair (sbp) member associated, via at least one covalent or non-covalent bond, with i) a photosensitizer, capable in its excited state of activating oxygen to its singlet state, and ii) a suspendible particle;
• and
• (b) a second composition comprising an sbp member associated, via at least one covalent or non-covalent bond, with i) a chemiluminescent compound, capable of chemiluminescence upon reaction with singlet oxygen, and ii) a suspendible particle.

A kit comprising:

• (a) a first composition comprising an antibody as a member of a specific binding pair (sbp) associated, via at least one covalent or non-covalent bond, with i) a phthalocyanine photosensitizer, which is capable in its excited state of activating oxygen to a singlet state, and ii) a suspendible latex particle;
• and
• (b) a second composition comprising an antibody as a sbp member associated, via at least one covalent or non-covalent bond, with i) an enol ether chemiluminescent compound, which is capable of chemiluminescence upon reaction with singlet oxygen, and ii) a suspendible latex particle.

A kit comprising, in packaged combination,

• a) a composition comprising a first suspendible particle, wherein said first suspendible particle comprises a chemiluminescent compound capable of emitting light upon interaction with singlet oxygen, and wherein said first suspendible particle is bound to a first specific binding pair (sbp) member, and
• b) a composition comprising a second suspendible particle, wherein said second suspendible particle comprises a photosensitizer capable, in its excited state, of activating oxygen to its singlet state, and wherein said second suspendible particle is bound to a second specific binding pair (sbp) member.