Dye treatment solution and photodynamic therapy and method of using same

A infection or sterilization treatment kit comprising:

• a volume of a concentration including a combination of polymyxin B and at least one of methylene blue or toluidene blue, the concentration ranging from about 10 μg/ml to about 500 μg/ml;
• and
• a light emitting treatment device having a thickness, one or more surfaces and one or more light dosage members disposed therein, the one or more light dosage members configured to emit light at an infection at wavelengths ranging from about 450 nm to about 850 nm;
• a dosage rate ranging from about 0 to about 150 mw/cm2;
• and a light dose ranging from 0 to about 300 J/cm2.

A method of treating an infection or sterilization comprising:

• providing one or more cells;
• disposing a concentration including a combination of polymyxin B and at least one of methylene blue or toluidene blue on the one or more cells;
• applying a light having a wavelength ranging from about 450 nm to about 850 nm;
• a dosage rate ranging from about 0 to about 150 mw/cm2;
• and a light dose ranging from 0 to about 300 J/cm2 to the one or more cells wherein the combination of light and dye is adapted to cause intracellular enzyme deactivation of the one or more cells.