Transcription factor and method for regulation of seed development, quality and stress-tolerance
AssigneeThe Regents of the University of California
InventorsRuth R. Finkelstein, Tim Lynch, Howard M. Goodman, Ming-Li Wang
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Abstract
Identification, cloning and sequencing of the Arabidopsis ABI4 gene involved in seed response to abscisic acid (ABA) that regulates production of seed nutrient reserves and desiccation protectants. A method for regulating seed development, viability, stress-tolerance and nutrient reserves.
Claims13
An isolated DNA sequence comprising a nucleotide sequence identified as SEQ ID NO:
- 1 or a mutant thereof comprising a nucleotide sequence identified as SEO ID NO:
- 3.
A recombinant nucleotide sequence comprising a nucleotide sequence depicting a mutant ABI4 gene set forth in SEQ ID NO:
- 3.
A method for increasing storage reserves in seeds and dessication protectants in vegetative tissue in a recipient seed or vegetative tissue by altering expression of the ABI4 gene, said method comprising steps:
- (a) constructing the ABI4 transgene by fusing ABI4 coding sequence comprising SEQ ID NO:
- 1 to a regulatory sequence for overexpression of said coding sequence in embryos or shoots derived from said recipient seed or vegetative tissue;
- (b) transferring the transgene constructed in step (a) into the recipient seed by Agrobacterium-mediated transformation, by biolistic transformation, or by electroporation;
- and
- (c) selecting a transgenic individual overexpressing said coding sequence from primary transformant lines by scoring for resistance to antibiotics and herbicides.
A method for production of a recombinant transgenic seed or vegetative tissue having increased storage reserves and dessication protectants, said method comprising a step of overexpressing a protein comprising an amino acid sequence identified as SEQ ID NO:
- 2 in a seed or vegetative tissue infiltrated with a culture of Agrobacterium tumefaciens transformed with a plasmid comprising a nucleotide sequence encoding SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.