Reagent cocktail preparation for the rapid production of serum
AssigneeInternational Technidyne Corporation
InventorFrank M. LaDuca
Life of this patent
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Abstract
A cocktail reagent preparation for the rapid production of serum contains thrombin, snake venom, and protamine sulfate. The preparation employs very small quantities of clot promoting substances which behave in a synergistic manner such that rapid clotting of highly heparinized blood is achieved without altering the chemical analysis of the blood enzymes, proteins, sugars, or electrolytes. Thus, clinicians who rely upon the results of such tests can more closely monitor organ and tissue function and adjust patient therapies accordingly.
Claims24
A reagent preparation for the rapid production of serum when said preparation is mixed with a given volume of blood, wherein said mixture includes thrombin in a concentration range of about 0.2-3.0 units per ml, snake venom having a concentration in the range of about 0.005-0.2 mg per ml, and a heparin neutralizing substance including a protamine salt having a concentration in the range of about 0.02-0.08 mg per ml, said preparation causing said blood to rapidly clot to enable one to obtain a serum providing clinically accurate blood chemistry analysis.
A cocktail preparation to enable relatively rapid clotting of highly heparinized blood when said cocktail is mixed with a given volume of blood, comprising thrombin, a heparin neutralizing substance, and a snake venom which snake venom is capable of converting fibrinogen to fibrin and is unaffected by the presence of heparin, said thrombin, heparin neutralizing substance, and snake venom present in sufficient amounts to rapidly clot said highly heparinized blood sample and to enable the serum to be separated from said clotted matter, said serum to be chemically tested to provide clinically accurate blood chemistry results.
A method of preparing a cocktail reagent for rapidly producing serum from blood, comprising;
- preparing a first solution by dissolving bovine thrombin in barbital buffered saline to a concentration of 100 units per ml;
- preparing a second solution by dissolving a snake venom in barbital buffered saline to a concentration of 1 mg per ml;
- preparing a third solution by dissolving protamine sulfate in a sodium chloride solution to a concentration of 10 mg per ml;
- and
- combining a predetermined amount of said first, said second and said third solutions to form a mixture such that the final concentration of said mixture contains 0.5 units thrombin per ml, 0.01 mg snake venom per ml, and 0.05 mg protamine sulfate per ml.
Dependencies are inferred from the claim text.