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US 6,248,535 B1Granted
Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
AssigneeUniversity of Southern California
InventorsKathleen Danenberg, Peter V. Danenberg, Steven Swenson
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Abstract
Methods are disclosed for rapid, reliable and simple isolation of RNA from formalin-fixed paraffin-embedded tissue samples. RNA purified in this manner can be used to monitor gene expression levels. The tissue sample can be a tumor or other pathological tissue.
Claims32
A method for recovering DNA, RNA or proteins from a formalin-fixed paraffin-embedded biological tissue sample comprising:
- deparaffinizing the sample;
- heating the sample in a chaotropic solution comprising an effective concentration of a guanidinium compound to a temperature in the range of about 60 to about 100° C. for a time period of about 30 to about 120 minutes;
- and
- recovering said RNA, DNA or protein from said chaotropic solution.
1 more element
A method for isolating RNA from a formalin-fixed paraffin-embedded biological tissue sample comprising:
- deparaffinizing the sample;
- heating the sample in a chaotropic solution comprising an effective concentration of a guanidinium compound to a temperature in the range of about 75 to about 100° C. for a time period of about 30 to about 60 minutes;
- and
- recovering said RNA from said chaotropic solution.
1 more element
Dependencies are inferred from the claim text.