Parasitic helminth cuticlin nucleic acid molecules and uses thereof
OwnerRamaswamy Chandrashekar, Tony H. Moralesinventors
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Abstract
The present invention relates to: parasitic helminth cuticlin proteins; parasitic helminth cuticlin nucleic acid molecules, including those that encode such cuticlin proteins; antibodies raised against such cuticlin proteins; and compounds that inhibit parasitic helminth cuticlin activity. The present invention also includes methods to obtain such proteins, nucleic acid molecules, antibodies, and inhibitory compounds. Also included in the present invention are therapeutic compositions comprising such proteins, nucleic acid molecules, antibodies and/or inhibitory compounds as well as the use of such therapeutic compositions to protect animals from diseases caused by parasitic helminths.
Claims7
An isolated Dirofilaria immitis nucleic acid molecule, that encodes a cuticlin protein or that is complementary to a nucleic acid molecule which encodes a cuticlin protein wherein said Dirofilaria immitis nucleic acid molecule hybridizes in a solution comprising 17.53 grams of sodium chloride and 8.82 grams sodium citrate in 0.1 liters of water, pH 7 (2×SSC) in the absence of nucleic acid helix destabilizing agents, at a temperature of 37° C., and washing in a solution comprising 8.765 grams of sodium chloride and 4.41 grams sodium citrate in 0.05 liters of water, pH 7 (1×SSC) in the absence of nucleic acid helix destabilizing agents at a temperature of 64° C., to a nucleic acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, and SEQ ID NO:5.
Dependencies are inferred from the claim text.