Matrix attachment regions
AssigneeNorth Carolina State University
InventorsSusan Michalowski, Steven Spiker
Life of this patent
Estimating the term…
Abstract
Matrix attachment regions isolated from a higher plants, and DNA constructs and vectors containing such matrix attachment regions, are described. A method of identifying matrix attachment regions is provided.
Claims19
An isolated DNA molecule having a nucleotide sequence selected from the group consisting of:
- (a) SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11 and SEQ ID NO:13;
- and
- (b) sequences that hybridize to the isolated DNA of (a) above under conditions represented by a wash stringency of 0.3M NaCl, 0.03M sodium citrate, and 0.1% SDS at 60° C., and which encode a matrix attachment region.
A DNA construct comprising a transcription initiation region, a structural gene positioned 3′ to said transcription initiation region and operatively associated therewith, and a matrix attachment region positioned either 5′ to said transcription initiation region or 3′ to said structural gene, wherein said matrix attachment region has a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11 and SEQ ID NO:13;
- said DNA construct carried by a plant transformation vector.
A recombinant tobacco plant comprising transformed tobacco plant cells, said transformed tobacco plant cells containing a heterologous DNA construct comprising a transcription initiation region functional in plant cells, a structural gene positioned 3′ to said transcription initiation region and operatively associated therewith, and a matrix attachment region positioned either 5′ to said transcription initiation region or 3′ to said structural gene,
- wherein said matrix attachment region has a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11 and SEQ ID NO:13.
Dependencies are inferred from the claim text.