Production of virus and purification of viral envelope proteins for vaccine use
AssigneeConnaught Laboratories Limited
InventorsMary Elizabeth Ewasyshyn, Barry Ian Caplan, Anne-Marie Bonneau, Michel Henri Klein
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Abstract
Immunogenic envelope glycoproteins are produced from enveloped virus, such as of the paramyxoviridae family, particularly PIV-3 and RSV, by culturing the virus in the substantial absence of exogenous serum proteins, isolating the virus from the tissue culture, solubilizing the envelope glycoproteins and isolating the solubilized envelope glycoproteins by chromatography.
Claims8
A method of isolating immunogenic envelope glycoproteins of a virus belonging to the paramyxoviridae family, wherein said glycoproteins are not denatured by exposure to acidic conditions and/or chaotropic agents and which are protective, highly-immunogenic and non-immunopotentiating, which comprises:
- growing said virus in a culture medium which is essentially free from exogenous growth factors and serum proteins on a cell substrate which is a diploid or continuous cell line,
- isolating said virus from tissue culture,
- solubilizing the envelope glycoproteins from said isolated virus, and
- isolating and co-purifying said solubilized envelope glycoproteins by affinity chromatography.
Dependencies are inferred from the claim text.