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US 6,242,252 B1Granted
Hepatic progenitors and method of isolating same
AssigneeAlbert Einstein College of Medicine of Yeshiva University
InventorsLola M. Reid, Samuel H. Sigal, Shlomo Brill, Patricia A. Holst
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Abstract
This invention relates to methods of isolating hepatic progenitors utilizing panning techniques and fluorescence activated cell sorting. This invention further relates to isolated hepatic progenitors and to a method of treating liver dysfunction as well as to methods of forming artificial livers.
Claims32
A method of isolating hepatic progenitors from liver comprising:
- (a) preparing a single cell suspension of liver cells;
- (b) panning said suspension utilizing antibodies specific for hemopoietic cells, mesenchymal cells, mature liver cells, or combinations thereof, to remove said hemopoietic cells, mesenchymal cells, and mature liver cells from said suspension;
- (c) performing fluorescence activated cell sorting utilizing said antibodies so as to remove hemopoietic cells, mesenchymal cells, and mature liver cells, from said cell suspension;
- and
- (d) performing multiparametric fluorescence activated cell sorting on said suspension utilizing at least one antibody to a hepatic cell marker, side scatter forward scatter, autofluorescence, or combinations thereof, such that the cells remaining in said suspension are enriched hepatic progenitors.
2 more elements
A method of isolating hepatic progenitors from liver comprising:
- (a) preparing a single cell suspension of liver cells;
- and
- (b) panning said suspension utilizing antibodies specific for hemopoietic cells, mesenchymal cells, mature liver cells, or combinations thereof, to remove said hemopoietic cells, mesenchymal cells, and mature liver cells, or combinations thereof, from said suspension such that the cells remaining in said suspension are enriched hepatic progenitors.
Dependencies are inferred from the claim text.