Immunostimulatory nucleic acid molecules
AssigneesUniversity of Iowa Research Foundation, Coley Pharmaceutical Group, Inc., The United States of America as represented by the Department of Health and Human Services
InventorsArthur M. Krieg, Joel N. Kline
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Abstract
Nucleic acid sequences containing unmethylated CpG dinucleotides that modulate an immune response including stimulating a Th1 pattern of immune activation, cytokine production, NK lytic activity, and B cell proliferation are disclosed. The sequences are also useful a synthetic adjuvant.
Claims49
A method for inducing Il-6 in a subject comprising
- administering to the subject an effective amount to induce Il-6 in the subject of an immunostimulatory nucleic acid, having a sequence comprising:
- 5′X1X2CGX3X3′
- wherein C is unmethylated, wherein X1, X2 and X3, X4 are nucleotides, and wherein the 5′ X1 X2CGX3 X4 3′ sequence is a non-palindromic sequence.
A method of stimulating natural killer cell lytic activity comprising exposing a natural killer cell to an immunostimulatory nucleic acid to stimulate natural killer cell lytic activity, the immunostimulatory nucleic acid having a sequence comprising:
- 5′X1X2CGX3X3′
- wherein C is unmethylated, wherein X1X2 and X3X4 are nucleotides, and wherein the 5′X1X2CGX3 X43′ sequence is a non-palindromic sequence.
A method for inducing interferon-gamma in a subject to treat an immune system deficiency, comprising:
- administering to a subject having an immune system deficiency an effective amount to induce interferon-gamma production in the subject of an immunostimulatory nucleic acid, having a sequence comprising:
- 5′X1X2CGX3X43′
- wherein C is unmethylated, wherein X1X2 and X3X4 are nucleotides, and wherein the sequence of the formula X1X2CGX3X4 is not palindromic.
A method for inducing Il-12 in a subject comprising:
- administering to the subject an effective amount to induce Il-12 in the subject, of an immunostimulatory nucleic acid having a sequence comprising:
- 5′X1X2CGX3X3′
- wherein C is unmethylated, wherein X1, X2, X3, and X4 are nucleotides, and wherein the sequence of the formula X1X2CGX3 X4 is not palindromic.
Dependencies are inferred from the claim text.