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US 6,238,565 B1Granted
Monolithic matrix for separating bio-organic molecules
AssigneeVarian, Inc.
InventorRichard Hatch
Life of this patent
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Abstract
The present invention provides monolithic polymer matrices for the separation of bio-organic molecules by liquid chromatography. In one embodiment, the matrix is formed from a polymerization mixture including (i) a hydrophobic monomer, (ii) a crosslinking agent, and (iii) a porogenic solvent or mixture of porogenic components. The monolithic matrices of the invention are particularly useful for resolving polynucleotides (e.g., DNA and/or RNA) in samples by way of reversed-phase ion-pairing chromatography.
Claims14
A method for resolving a mixture containing at least one polynucleotide, comprising:
- applying said mixture to a porous monolithic polymer matrix held in a stationary fashion by a support,
- wherein said monolithic polymer matrix is formed from polymerization of a monomer, or combination of monomers, selected from C3 to C30 alkyl methacrylates, in the presence of a crosslinking agent and a porogenic solvent, and
- separating said mixture by ion-pair reverse-phase chromatography, by passing an eluant containing an ion-pairing agent through the monolithic matrix.
Dependencies are inferred from the claim text.