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AssigneeUniversity of Massachusetts, Amherst Campus
InventorsSteven L. Stice, Jose Cibelli, James Robl, Paul Golueke, F. Abel Ponce de Leon, D. Joseph Jerry
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An improved method of nuclear transfer involving the transplantation of donor differentiated cell nuclei into enucleated oocytes of the same species as the donor cell is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring, and for production of isogenic CICM cells, including human isogenic embryonic or stem cells. Production of genetically engineered or transgenic mammalian embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.
A method for producing a mammalian cultured inner cell mass (CICM) cell line by nuclear transfer comprising the following steps:
A method of producing a non-human mammalian embryo by nuclear transfer comprising transplantation of a non-human mammalian cell or a nucleus of a non-human mammalian cell into an enucleated oocyte of the same species as the donor cell or donor cell nucleus, activation of the recipient oocyte containing the donor cell or donor cell nucleus, and incubation of the activated oocyte to produce an embryo, wherein the donor cell is a proliferating mammalian differentiated cell or wherein the donor nucleus is from a proliferating mammalian differentiated cell.
Dependencies are inferred from the claim text.