Biocatalysts with amine acylase activity
AssigneeNovartis AG
InventorsOreste Ghisalba, Matthias Kittelmann, Kurt Laumen, Paula Walser-Volken
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Abstract
The invention belongs to the field of biotechnology. It concerns a biocatalyst, i.e. a dead or living microorganism or a polypeptide, preferably in isolated form, which exhibits acylase enzymatic activity without lipase- or esterase-activity. The biocatalyst is capable of stereoselectively hydrolysing a racemic acylamide which has an aliphatic acyl residue and which is not a derivative of a natural amino acid.
Claims8
A purified biocatalyst isolated from a microorganism selected from the group consisting of Rhodococcus globerulus, Rhodococcus equi and Arthrobacter aurescens, wherein the biocatalyst exhibits amine acylase enzymatic activity without lipase- or esterase-activity, and wherein the biocatalyst stereoselectively hydrolyses a racemic acylamide of formula (1)
- wherein R2 is aryl or C1-C4aryl;
- or R1 and R2 together are forming a 5 to 7 membered ring substituted by or fused to aryl;
- R3 is an aliphatic acyl residue;
- and
- R1 and R3 together are chosen in order to form a compound of formula (1) that is not a derivative of a natural amino acid;
- wherein each of the residues can be substituted or unsubstituted.
A purified biocatalyst exhibiting amine acylase enzymatic activity without lipase- or esterase-activity, wherein the biocatalyst stereoselectively hydrolyses a racemic acylamide of formula (1)
- wherein R2 is aryl or C1-C4aryl;
- or R1 and R2 together are forming a 5 to 7 membered ring substituted by or fused to aryl;
- R3 is an aliphatic acyl residue;
- and
- R1 and R3 together are chosen in order to form a compound of formula (1) that is not a derivative of a natural amino acid;
- wherein each of the residues can be substituted or unsubstituted, wherein the biocatalyst comprises an amino acid sequence selected from the group consisting of SEQ ID Nos 1, 2, 3 and 4.
Dependencies are inferred from the claim text.