Maize A3 promoter and methods for use thereof
AssigneeDekalb Genetics Corporation
InventorsDavid McElroy, Alan L. Kriz, Emil M. Orozco, Jr., Matt Griffor
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Abstract
The current invention provides the maize A3 promoter and actin 2 intron. Compositions comprising these sequences are described, as well as transformation constructs derived therefrom. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the A3 promoter directly by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.
Claims63
An isolated nucleic acid comprising a maize A3 promoter.
An isolated maize A3 promoter isolatable from the nucleic acid sequence of SEQ ID NO:4.
An expression vector comprising a maize A3 promoter operably linked to a selected DNA sequence.
A transgenic plant stably transformed with a selected DNA comprising a maize A3 promoter.
A method of expressing a selected DNA in a transgenic plant comprising the steps of:
- (i) obtaining a construct comprising a selected coding region operably linked to a maize A3 promoter;
- (ii) transforming a recipient plant cell with said construct;
- and
- (iii) regenerating a transgenic plant expressing said selected coding region from said recipient plant cell.
A transgenic plant cell stably transformed with a selected DNA comprising a maize A3 promoter.
Dependencies are inferred from the claim text.