Tenascin-C nucleic acid ligands
AssigneeGilead Sciences, Inc.
InventorsBrian Hicke, Stephen Warren, David Parma, Larry Gold
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Abstract
Methods are described for the identification and preparation of nucleic acid ligands to tenascin-C. Included in the invention are specific RNA ligands to tenascin-C identified by the SELEX method. Further included in the invention are methods for detecting the presence of a disease condition in a biological tissue in which tenascin-C is expressed.
Claims14
A nucleic acid ligand to tenascin-C identified according to the method comprising:
- a) contacting a candidate mixture of nucleic acids with tenascin-C, wherein nucleic acids having an increased affinity to tenascin-C relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;
- b) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture;
- c) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acids with relatively higher affinity and specificity for binding to tenascin-C, whereby a nucleic acid ligand of tenascin-C may be identified.
A purified and isolated non-naturally occurring nucleic acid ligand to tenascin-C.
A method of identifying nucleic acid ligands to tenascin-C, the method comprising:
- a) contacting a candidate mixture of nucleic acids with tenascin-C, wherein nucleic acids having an increased affinity to tenascin-C relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;
- b) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture;
- c) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acids with relatively higher affinity and specificity for binding to tenascin-C, whereby a nucleic acid ligand of tenascin-C may be identified.
Dependencies are inferred from the claim text.