Cytokinin oxidase
AssigneeThe Curators of the University of Missouri
InventorRoy O. Morris
Life of this patent
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Abstract
An isolated protein which exhibits cytokinin oxidizing activity selected from the group consisting of SEQ. ID No. 1, a protein having an amino acid sequence which includes the amino acid sequence of SEQ. ID No. 1, a protein having an amino acid sequence which includes a portion of the amino acid sequence of SEQ. ID No. 1, the included portion being at least about 20 amino acid residues in length and conferring the cytokinin oxidizing activity on the protein, and a protein including an amino acid sequence with at least about 65% sequence identity to SEQ. ID No. 1, the remainder of amino acid residues being conservatively substituted. Nucleic acids encoding proteins which exhibit cytokinin oxidizing activity and related products and methods are also disclosed.
Claims20
An isolated nucleic acid polymer encoding a protein which exhibits cytokinin oxidizing activity, selected from the group consisting of:
- (a) SEQ. ID NO. 1,
- (b) a protein having an amino acid sequence which includes the amino acid sequence of SEQ. ID NO. 1, and
- (c) a protein including an amino acid sequence wherein at least 65% of the amino acids of the amino acid sequence correspond to the amino acid sequence of SEQ. ID NO. 1.
An isolated nucleic acid polymer which encodes a protein which exhibits cytokinin oxidizing activity, wherein the nucleic acid polymer is selected from the group consisting of:
- (a) SEQ. ID NO. 2,
- (b) SEQ. ID NO. 3,
- (c) a nucleic acid polymer with a sequence described by SEQ. ID NO. 10,
- (d) a nucleic acid polymer whose sequence contains a sequence selected from the group consisting of SEQ. ID NO. 2, SEQ. ID NO. 3, or a nucleic acid polymer with a sequence described by SEQ. ID NO. 10,
- (e) a nucleic acid polymer which hybridizes to a nucleotide sequence selected from the group consisting of SEQ. ID NO. 2, SEQ. ID NO. 3, and a nucleic acid polymer with a sequence described by SEQ. ID NO. 10, under a wash stringency of 0.5× to 2× SSC buffer, 0.1% SDS, at 55-65° C., and
- (f) a nucleic acid polymer having a nucleotide sequence complementary to any of the nucleic acid sequences (a)-(e).
An isolated nucleic acid polymer which encodes a protein which exhibits cytokinin oxidizing activity, wherein the nucleic acid polymer is selected from the group consisting of:
- (a) a nucleic acid polymer which encodes a protein comprising an amino acid sequence of at least 20 amino acids, wherein 90% of the amino acids in said amino acid sequence are the same as the corresponding amino acids of a portion of SEQ. ID NO. 1 of the same size, and
- (b) a nucleic acid polymer having a nucleotide sequence complementary to a nucleic acid sequence of (a).
Dependencies are inferred from the claim text.