Enzymes with xylanase activity from aspergillus aculeatus
AssigneeNovo Nordisk A/S
InventorsLene Venke Kofod, MArkus Sakari Kauppinen, Stephan Christgau, Hans Peter Heldt-Hansen, Henrik Dalbøge, Lene Nonboe Andersen, Joan Qi Si, Tina Sejersgåard Jacobsen, Niels Munk, Anette Müllertz
Life of this patent
Estimating the term…
Abstract
An enzyme preparation comprising (a) first enzyme with xylanase activity and (b) a second enzyme having cellulotytic, xylanolytic or pectinolytic activity and are supported by the specification.
Claims20
An enzyme preparation comprising (a) an essentially pure enzym having xylanase activity, as measured by release of reducing sugars from birch xylan or by release of blue color from AZCL-Birch xylan, said enzyme having a pH optimum of 4-5 and a temperature optimum of 70-80° C., which is encoded by a DNA sequence which hybridizes to a DNA sequence depicted in SEQ ID NO. 3 under the following conditions:
- hybridizing in 5×SSC, 5×Denhardt's solution, 50 mM sodium phosphate, pH 6.8 and 50 ug denatured sonicated calf thymus DNA for 18 hrs. at about 40° C. followed by warming tbree times in 2×SSC, 0.2% SDS at 40° C. for 30 minutes and (b) a second enzyme having cellulolytic, xylanolytic or pectinolytic activity.
An enzyme preparation comprising an enzyme having xylanase activity, as measured by release of reducing sugars from birch xylan or by release of blue color from AZCL-Birch xylan, said enzyme has a pH optimum of 4-5;
- (b) a temperature optimum of 70-80° C.;
- (c) is encoded by a DNA sequence comprising thc partial DNA sequnce depicted in SEQ ID NO. 41;
- (d) is derivable from a strain of Aspergillus and (b) a second enzyme having cellulytic, xylanolytic or pectinolytic activity.
Dependencies are inferred from the claim text.