Sarcosine oxidase and process for producing the same
AssigneeKikkoman Corporation
InventorsToshio Ichikawa, Yasuji Koyama
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Abstract
This invention relates to sarcosine oxidase having the following physico-chemical properties: (a) action: oxidatively hydrolyzing 1 mole sarcosine to give 1 mole glycine, 1 mole formaldehyde, and 1 mole hydrogen peroxide; (b) substrate specificity: specific for sarcosine; (c) optimum pH: 7.0-8.0; (d) stable pH range: 7.0-9.5; (e) suitable temperature range for action: 50° C.; (f) thermostability: 55° C. or less; and (g) molecular weight: 44,000 daltons (when estimated roughly from the amino acid sequence of the wild-type), and to a process for producing the sarcosine oxidase, comprising the steps of culturing a microorganism having an ability to produce the sarcosine oxidase and collecting the sarcosine oxidase from the culture are provided.
Claims4
An isolated mutant sarcosine oxidase derived from randomly mutating the DNA sequence encoding the wild-type sarcosine oxidase of Bacillus sp. NS-129 of SEQ ID NO:
- 1, wherein the mutant sarcosine oxidase has the following physico-chemical properties:
- (a) action:
- oxidatively hydrolyzing 1 mole sarcosine to give 1 mole glycine, 1 mole formaldehyde, and 1 mol hydrogen peroxide;
- (b) substrate specificity:
- specific for sarcosine;
- (c) optimum pH:
- 7.0-8.0;
- (d) stable pH range:
- 7.0-9.5;
- (e) suitable temperature for action:
- 50° C.;
- and
- (f) thermostability:
- 55° C. or less for at least 10 minutes.
Dependencies are inferred from the claim text.