Multimeric hybrid gene encoding a chimeric protein which confers protection against parainfluenza virus and respiratory synctial virus
AssigneeConnaught Laboratories Limited
InventorsMichel H. Klein, Run-Pan Du, Mary E. Ewasyshyn
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Abstract
Multimeric hybrid genes encoding the corresponding chimeric protein comprise a gene sequence coding for an antigenic region of a protein from a first pathogen linked to a gene sequence coding for an antigenic region of a protein from a second pathogen. The pathogens particularly are parainfluenza virus (PIV) and respiratory syncytial virus (RSV). A single recombinant immunogen is capable of protecting infants and similar susceptible individuals against diseases caused by both PIV and RSV.
Claims20
A multimeric hybrid gene encoding a chimeric protein, said hybrid gene being selected from the group consisting of:
- (a) a hybrid gene comprising a nucleotide sequence coding for a native PIV-3 F protein linked to a nucleotide sequence coding for a native RSV G protein;
- (b) a hybrid gene comprising a nucleotide sequence coding for a native PIV-3 F protein linked to a nucleotide sequence coding for a native RSV F protein;
- (c) a hybrid gene comprising a nucleotide sequence coding for a native PIV-3 HN protein linked to a nucleotide sequence coding for a native RSV G protein;
- and
- (d) a hybrid gene comprising a nucleotide sequence coding for a native PIV-3 HN protein linked to a nucleotide sequence coding for a native RSV F protein.
A process for preparation of a chimeric protein, which comprises:
- isolating a first nucleotide sequence coding for a native PIV-3 F protein or a first nucleotide sequence coding for a native PIV-3 HN protein,
- isolating a second nucleotide sequence coding for a native RSV G protein or a second nucleotide sequence coding for a native RSV F protein,
- linking said first and second nucleotide sequences to form a multimeric hybrid gene, said multimeric hybrid gene being selected from the group consisting of:
- (a) a hybrid gene comprising a nucleotide sequence coding for a native PIV-3F protein linked to a nucleotide sequence coding for a native RSV G protein;
- (b) a hybrid gene comprising a nucleotide sequence coding for a native PIV-3 F protein linked to a nucleotide sequence coding for a native RSV F protein;
- (c) a hybrid gene comprising a nucleotide sequence coding for a native PIV-3 HN protein linked to a nucleotide sequence coding for a native RSV G protein;
- and
- (d) a hybrid gene comprising a nucleotide sequence coding for a native PIV-3 HN protein linked to a nucleotide sequence coding for a native RSV F protein;
- and
- expressing the multimeric hybrid gene in a eukaryotic cellular expression system.
Dependencies are inferred from the claim text.