Gene encoding a putative efflux protein for solvents/antibiotics in pseudomonas mendocina
AssigneeE. I. Du Pont de Nemours and Company
InventorKevin Keqin Chen
Life of this patent
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Abstract
This invention relates to the isolation of a novel putative efflux gene from Pseudomonas mendocina. The putative efflux gene is useful for probing an organism's efflux system to gain an understanding of the mechanisms of solvent tolerance. The invention further provides a Pseudomonas mendocina strain deficient in this gene. This strain is unable to grow in the presence of chloramphenicol and, compared to the wildtype strain, grows slowly in the presence of high concentrations of PHBA.
Claims9
An isolated nucleic acid molecule encoding an efflux protein from Pseudomonas selected from the group comprising of:
- (a) an isolated nucleic acid molecule encoding the amino acid sequence set forth in SEQ NO:2;
- (b) an isolated nucleic acid molecule that hybridizes to the complement of the isolated nucleic acid molecule of (a) under the following hybridization conditions:
- 0.1×SSC, 0.1% SDS at 65° C. and washed with 2×SSC, 0.1% SDS followed by a second wash in 0.2×SSC, 0.1% SDS.
An isolated nucleic acid molecule that is complementary to the full length of a nucleic acid molecule selected from the group consisting of:
- (a) an isolated nucleic acid molecule encoding the amino acid sequence set forth in SEQ NO:2;
- (b) an isolated nucleic acid molecule that hybridizes to the complement of the isolated nucleic acid molecule of (a) under the following hybridization conditions:
- 0.1×SSC, 0.1% SDS at 65° C. and washed with 2×SSC, 0.1% SDS followed by a second wash in 0.2×SSC, 0.1% SDS.
Dependencies are inferred from the claim text.