Baculovirus expression system and method for high throughput expression of genetic material
AssigneeOnyx Pharmaceuticals, Inc.
InventorsRobin Clark, Anthony Davies
Life of this patent
Estimating the term…
Abstract
The present invention provides novel recombinant baculovirus expression systems for expressing foreign genetic material in a host cell. Such expression systems are readily adapted to an automated method for expression foreign genetic material in a high throughput manner. In other aspects, the present invention features a novel automated method for determining the function of foreign genetic material by transfecting the same into a host by way of the recombinant baculovirus expression systems according to the present invention.
Claims22
A baculovirus expression system comprising at least one selectable marker gene, a promoter, a transcription termination sequence, a nucleotide sequence that encodes a tag, and a cloning site operable to accept foreign cDNA genetic material wherein said foreign genetic material is operably linked to said promoter, and said tag is expressed as part of said cDNA.
A method for producing a recombinant baculovirus expression system comprising the steps of
- (a) providing a baculovirus transfer vector comprising at least one selectable marker gene, a promoter such as a polyhedrin promoter of a polyhedrin structural gene or a fragment thereof, a transcription termination sequence such as a transcription termination sequence of a polyhedrin structural gene or fragment thereof, a nucleotide sequence that encodes a tag, a cloning site operable to accept foreign cDNA genetic material, wherein said foreign genetic material is operably linked to said promoter, and said tag is expressed as part of said cDNA;
- and
- (b) ligating foreign genetic material into the baculovirus transfer vector at the cloning site such that the foreign genetic material is under the control of the promoter.
A method for expressing foreign genetic material comprising the steps of:
- (a) providing a baculovirus transfer vector comprising an apoptosis suppressor gene, a promoter such as a polyhedrin promoter of a polyhedrin structural gene or a fragment thereof, a transcription termination sequence such as a transcription termination sequence of a polyhedrin structural gene or fragment thereof, a nucleotide sequence that encodes a tag, a cloning site operable to accept foreign cDNA genetic material, wherein said foreign genetic material is operably linked to said promoter, and said tag is expressed as part of said cDNA;
- (b) ligating foreign genetic material into the baculovirus transfer vector at the cloning site such that the foreign genetic material is under the control of the promoter;
- and
- (c) transfecting the baculovirus transfer vector comprising the foreign genetic material into a host cell.
A method for determining the function of foreign genetic material comprising the steps of:
- (a) providing a baculovirus transfer vector comprising an apoptosis suppressor gene, a promoter such as a polyhedrin promoter of a polyhedrin structural gene or a fragment thereof, a transcription termination sequence such as a transcription termination sequence of a polyhedrin structural gene or fragment thereof, a nucleotide sequence that encodes a tag, a cloning site operable to accept foreign cDNA genetic material, wherein said foreign genetic material is operably linked to said promoter, and said tag is expressed as part of said cDNA;
- (b) ligating foreign genetic material into the baculovirus transfer vector at the cloning site such that the foreign genetic material is under the control of the promoter;
- (c) transfecting the baculovirus transfer vector comprising the foreign genetic material into a host cell;
- and
- (d) observing the biological results produced thereby.
Dependencies are inferred from the claim text.