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US 6,214,577 B1Granted
Yeast vectors conferring antibiotic resistance
OwnerRobert Rogers Yocuminventor
Life of this patent
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Abstract
A vector having a gene for resistance to an antibiotic otherwise capable of killing a host yeast cell, the gene being transcribed from a yeast promoter sequence.
Claims12
An isolated DNA sequence comprising:
- a) a gene encoding resistance to an antibiotic, wherein said antibiotic inhibits growth of an untransformed host yeast cell,
- b) a promotor sequence that is not naturally associated with said gene encoding antibiotic resistance, wherein said antibiotic resistance gene is transcribed from said promotor sequence, such that said DNA sequence provides for direct selection for said DNA sequence upon transformation into said host yeast cell, wherein the first selective medium encountered by the transformed cells subsequent to transformation contains said antibiotic, and wherein said promotor sequence is capable of transcribing said antibiotic resistant gene such that said isolated DNA provides for said direct selection upon integration of said DNA sequence into a chromosome of said host yeast cell, and
- c) a DNA sequence that is homologous to a portion of a chromosome of said host yeast cell.
The vector pRY253, shown in FIG. 7.
The vector pRY255, shown in FIG. 8.
The vector pRY255A.
Dependencies are inferred from the claim text.