Method and kit for rapid preparation of autologous tissue medical devices
AssigneeMures Cardiovascular Research, Inc.
InventorsDan Simionescu, Agneta Simionescu
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Abstract
A method for the rapid chemical treatment of autologous connective tissues which enhances biocompatibility by reducing cytotoxicity, immunogenicity and calcification without impairing the mechanical properties of the fixed tissue. The procedure includes a short exposure of the autologous biological tissue to a dialdehyde fixative followed by the rapid neutralization of excess aldehyde with an aminoacid solution.
Claims16
A method for rapid fixation and neutralization of autologous biological tissue that renders the tissue suitable for animal and human implantation comprising:
- treating a animal or human tissue with an effective amount of glutaraldehyde which promotes tissue crosslinking;
- and
- treating the animal or human tissue with an unbranched alpha amino acid solution.
A method for the rapid chemical treatment of autologous connective tissues which enhances biocompatibility by reducing cytotoxicity, immunogenicity and calcification without impairing the mechanical properties of the tissue comprising:
- exposing the autologous connective tissue to a dialdehyde fixative for about 8 to about 15 minutes;
- and
- rapidly neutralizing excess aldehyde on the tissue with an unbranched alpha aminoacid solution.
A method for rapid fixation and neutralization of autologous biological tissue that renders the tissue suitable for animal and human implantation comprising:
- treating a animal or human tissue with an effective amount of glutaraldehyde which promotes tissue crosslinking for a period of about 8 to about 15 minutes;
- and
- treating the animal or human tissue with an unbranched alpha aminoacid solution having a pH of about 7.4 for a period of about 8 to about 30 minutes.
A method for the rapid chemical treatment of autologous connective tissues which enhances biocompatibility by reducing cytotoxicity, immunogenicity and calcification without impairing the mechanical properties of the autologous connective tissue comprising:
- exposing the autologous connective tissue to a dialdehyde fixative;
- and
- neutralizing excess aldehyde on the autologous connective tissue with an unbranched alpha aminoacid solution for about 8 to about 30 minutes.
Dependencies are inferred from the claim text.