Nucleic acid molecules from plants coding enzymes which participate in the starch synthesis
AssigneePlantTec Biotechnologie GmbH
InventorsJens Kossmann, Claus Frohberg
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Abstract
Nucleic acid molecules encoding enzymes which participate in the starch synthesis in plants are described. These enzymes are a novel isotype of starch synthase. Furthermore, the invention relates to vectors and host cells which were transformed with the described nucleic acid molecules, in particular to transformed plant cells and to plants which may be regenerated therefrom and which exhibit an increased or reduced activity of the described proteins.
Claims31
An isolated nucleic acid molecule comprising a nucleic acid sequence encoding a protein with a biological activity of a starch synthase, wherein the nucleic acid sequence is selected from the group consisting of:
- (a) a nucleic acid sequence encoding a protein comprising the amino acid sequence of SEQ ID NO:
- 2;
- (b) the coding region of SEQ ID NO:
- 1;
- (c) a nucleic acid sequence that has 60% or greater sequence identity to the nucleic acid sequence of (a) or (b) and encodes a protein with starch synthase activity;
- (d) a fragment of the nucleic acid sequence of (a), (b) or (c), wherein the fragment has the biological activity of a starch synthase;
- and
- (e) the respective complementary strand of the nucleic acid sequence of (a), (b), (c) or (d).
An isolated nucleic acid molecule, wherein said isolated nucleic acid molecule, when introduced into a plant cell, has a cosuppression effect on the expression of an endogenous gene in the plant cell, said endogenous gene encoding a protein comprising the amino acid sequence of SEQ ID NO:2, said isolated nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of:
- (a) a nucleic acid sequence encoding a protein comprising the amino acid sequence of SEQ ID NO:
- 2;
- (b) the coding region of SEQ ID NO:
- 1;
- (c) a nucleic acid sequence that has greater then 65% sequence identity to the nucleic acid sequence of (a) or (b);
- (d) a fragment of the nucleic acid sequence of (a), (b) or (c), wherein the fragment encodes a protein with the biological activity of a starch synthase;
- and
- (e) the respective complementary strand of the nucleic acid sense of (a), (b), (c) or (d).
Dependencies are inferred from the claim text.