Manipulation of Mlo genes to enhance disease resistance in plants
AssigneesPioneer Hi-Bred International, Inc., E.I. du Pont de Nemours and Company
InventorsCarl R. Simmons, Guo-Hua Miao
Life of this patent
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Abstract
Compositions and methods for enhancing disease resistance in plants are provided. The method involves modulating the activity of the Mlo3 sequences in the plant. Mlo3 sequences are provided that can be manipulated to enhance pathogen resistance in modified plants. Transformed plants, plant cells, tissues, and seed are also provided having enhanced disease resistance.
Claims16
A method for creating or enhancing disease resistance in a plant, said method comprising modulating the expression of an Mlo3 sequence wherein said sequence is selected from the group consisting of:
- (a) a nucleotide sequence comprising the maize Mlo3 sequence set forth in SEQ ID NO:
- 1;
- (b) a nucleotide sequence encoding the maize MLO3 amino acid sequence set forth in SEQ ID NO:
- 2;
- (c) a nucleotide sequence comprising an antisense sequence corresponding to a sequence of a) or b);
- (d) a nucleotide sequence encoding an Mlo3 protein and which hybridizes under high stringency conditions to one of the nucleotide sequences set forth in a), b) or c);
- and
- (e) a nucleotide sequence encoding an Mlo3 protein and comprising at least 80% sequence identity to the nucleotide sequence of a).
A modified plant having an altered level of an MLO3 protein wherein said plant has stably incorporated into its genome a DNA construct, wherein said DNA construct comprises a nucleotide sequence operably linked to a promoter capable of regulating transcription of said sequence in said plant, wherein said nucleotide sequence is selected from the group consisting of:
- (a) a nucleotide sequence encoding the maize MLO3 amino acid sequence set forth in SEQ ID NO:
- 2;
- (b) a nucleotide sequence comprising the maize Mlo3 sequences set forth in SEQ ID NO:
- 1;
- (c) a nucleotide sequence comprising an antisense sequence corresponding to a sequence of a) or b);
- and
- (d) a nucleotide sequence encoding an Mlo3 protein and comprising at least 80% sequence identity to at least one of the nucleotide sequences set forth in a), b) or c).
A modified plant cell having an altered level of an MLO3 protein wherein said plant cell has stably incorporated into its genome a DNA construct, wherein said DNA construct comprises a nucleotide sequence operably linked to a promoter capable of regulating transcription of said sequence in said plant cell, wherein said nucleotide sequence is selected from the group consisting of:
- (a) a nucleotide sequence comprising the maize Mlo3 gene set forth in SEQ ID NO:
- 1;
- (b) a nucleotide sequence coding the maize MLO3 amino acid sequence set forth in SEQ ID NO:
- 2;
- (c) a nucleotide sequence comprising an antisense sequence corresponding to a sequence of a) or b);
- and
- (d) a nucleotide sequence encoding an Mlo3 protein and comprising at least 80% sequence identity to at least one of the nucleotide sequences set forth in a), b) or c).
An isolated nucleotide sequence, said sequence comprising at least one of the nucleotide sequences selected from the group consisting of:
- (a) a nucleotide sequence comprising the maize Mlo3 gene set forth in SEQ ID NO:
- 1;
- (b) a nucleotide sequence encoding the maize MLO3 amino acid sequence set forth in SEQ ID NO:
- 2;
- (c) a nucleotide sequence comprising an antisense sequence corresponding to a sequence of a) or b);
- and
- (d) a nucleotide sequence encoding an Mlo3 protein and comprising at least 80% sequence identity to at least one of the nucleotide sequences set forth in a), b) or c).
Dependencies are inferred from the claim text.