DNA sequences coding for a cinnamoyl COA reductase, and applications thereof in the control of lignin contents in plants
AssigneesCentre National De La Recherche Scientifique, Institut National de la Recherche Agronomique
InventorsAlain-Michel Boudet, Magalle Pichon, Jacqueline Grima-Pettenati, Michel Beckert, Pascal Gamas, Jean-François Briat
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Abstract
The present invention relates to any DNA sequence comprising as a coding region all or part of the nucleotidic sequence coding for a mRNA coding coding for a cinnamoyl CoA reductase (CCR) in lucern and/or corn, or all or part of the nucleotide sequence complementary of the latter and coding for an antisense mRNA susceptible of hybridizing with said mRNA. The invention also relates to the use of said sequences for implementing processes for the regulation of lignin biosynthesis in plants.
Claims14
A method of producing transgenic plants within which the biosynthesis of lignins is regulated either in the sense of an increase, or in the sense of a reduction of the lignin levels produced, relative to the normal lignin levels produced in plants comprising:
- transforming plant cells with a recombinant nucleotidc sequence comprising one or more coding regions, wherein said coding regions are selected from the group consisting of:
- the nuclcotide sequence represented by SEQ ID NO:
- 1, coding for a mRNA, said mRNA coding for the Cinnamoyl CoA Reductase (CCR) of lucerne represented by SEQ ID NO:
- 2;
- and
- the nucleotide sequence represented by SEQ ID NO:
- 3, coding for a mRNA, said mRNA coding for the CCR of maize represented by SEQ ID NO:
- 4, and
- the nucleotide sequence fully complementary to that represented by SEQ ID NO:
- 1, or SEQ ID NO:
- 3.
An isolated DNA sequence comprising:
- the nuclcotide sequence represented by SEQ ID NO:
- 1, coding for a mRNA, said mRNA coding for the Cinnamoyl CoA Reductase (CCR) represented by SEQ ID NO:
- 2, or
- the nucleotide sequence represented by SEQ ID NO:
- 3, coding for a mRNA, said mRNA coding for a protein represented by SEQ ID NO:
- 4.
An isolated DNA sequence comprising:
- the nucleotide sequence fully complementary to that represented by SEQ ID NO:
- 1 or
- the nucleotide sequence fully complementary to that represented by SEQ ID NO:
- 3.
An isolated mRNA selected from
- the mRNA coded by the DNA sequence represented by SEQ ID NO:
- 1 and the mRNA coded by the DNA sequence represented by SEQ ID NO:
- 3.
An isolated nucleotide sequence coding for the CCRs represented by SEQ ID NO:
- 2 or SEQ ID NO:
- 4.
A plant, plant fragment, cell, fruit, seed, or pollen, transformed by incorporation of at least one nucleotide sequence selected from the group consisting of:
- the nucleotide sequence represented by SEQ ID NO:
- 1, coding for a mRNA, said mRNA coding for the Cinnamoyl CoA Reductase (CCR) of lucerne represented by SEQ ID NO:
- 2,
- the nucleotide sequence represented by SEQ ID NO:
- 3, coding for a mRNA, said mRNA coding for the CCR of maize represented by SEQ ID NO:
- 4, and
- the nucleotide sequence fully complementary to that represented by SEQ ID NO:
- 1 and SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.