Plant transcription regulators from circovirus

A genetic construct comprising an isolated SCSV promoter operable in a plant cell and at least one restriction endonuclease site downstream of said promoter to facilitate insertion of a heterologous gene operably linked to said promoter, wherein said SCSV promoter comprises a nucleotide sequence obtained from the group consisting of:

• (i) SEQ ID NO:
• 1 or a sequence complementary thereto;
• (ii ) SEQ ID NO:
• 3 or a secuence complementary thereto;
• (iii) SEQ ID NO:
• 4 or a sequence complementary thereto;
• (iv) SEQ ID NO:
• 5 or a sequence complementary thereto;
• (v) SEQ ID NO:
• 7 or a sequence complementary thereto;
• and
• (vi) a fraament of any one of (i) to (v), wherein said fragment is operable in a plant cell.

A genetic construct comprising at least two heterologous genes. wherein each of said heterologous genes is operably linked to an isolated nanovirus promoter operable in a plant cell, wherein said promoter is obtained from a seqment of the subterranean clover stunt virus (SCSV) genome selected from the group consisting of:

• (i) SCSV secment 1 (SEO ID NO:
• 1);
• (ii) SCSv segment 3 (SEO ID NO:
• 3);
• (iii) SCSV segment 4 (SEO ID NO:
• 4);
• (iv) SCSV seciment 5 (SEO ID NO:
• 5);
• (v) SCSV segment 7 (SEO ID NO:
• 7);
• (vi) a nucleotide seouence complementary to any one of (i) to (v);
• and
• (vii) a fragment of any one of (i) to (vi) which is operable in a plant cell.

A transgenic plant comprising an SCSV promoter introduced to said plant and a heterologous gene operably linked to said SCSV promoter, wherein said SCSV promoter comprises a nucleotide sequence obtained from the group consisting of:

• (i) SEQ ID NO:
• 1 or a sequence complementary thereto;
• (ii) SEQ ID NO:
• 3 or a sequence complementary thereto;
• (iii) SEQ ID NO:
• 4 or a sequence complementary thereto;
• (iv) SEQ ID NO:
• 5 or a sequence complementarv thereto;
• (v) SEQ ID NO:
• 7 or a sequence comtlementarv thereto:
• and
• (vi) a fragment of any one of (i) to (v), wherein said fragment is operable in a plant cell.

An isolated SCSV promoter sequence obtained from a nucleotide sequence selected from the group consisting of:

• SCSV segment 1 (SEQ ID NO:1);
• SCSV segment 3 (SEQ ID NO:3);
• SCSV segment 4 (SEQ ID NO:4);
• SCSV segment 5 (SEQ ID NO:5);
• SCSV segment 7 (SEQ ID NO:7);
• and a fragment of any one of SEQ ID NOS:
• 1, 3-5 or 7.

An isolated transcription terminator sequence of SCSV obtained from a nucleotide sequence selected from the group consisting of SCSV segment 1 (SEQ ID NO:1);

• SCSV segment 3 (SEQ ID NO:3);
• SCSV segment 4 (SEQ ID NO:4);
• SCSV segment 5 (SEQ ID NO:5);
• SCSV segment 7 (SEQ ID NO:7);
• and a transcription termination competent or polyadenylation competent fragment of any one of SEQ ID NOS:
• 1, 3-5 or 7.