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AssigneesNorth Carolina State University, The University of North Carolina at Chapel Hill
InventorsJoseph M. DeSimone, Ruben G. Carbonell, Stephanie A. Crette, Jonathan L. Kendall
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A method for the enzymatic production of a desired product in a liquid or supercritical carbon dioxide fluid. The method comprises: (a) providing a reaction mixture comprising at least one reaction substrate and a crosslinked enzyme crystal catalyst in a liquid or supercritical carbon dioxide fluid; and (b) reacting said substrate with said crosslinked enzyme crystal catalyst in said carbon dioxide fluid to produce said product. The reacting step is typically followed by the step of (c) separating the product from said reaction mixture. The crosslinked enzyme crystal catalyst preferably comprises an enzyme crystal crosslinked with a multifunctional crosslinking agent, said crosslinked enzyme crystal having resistance to exogeneous proteolysis, such that the crosslinked enzyme crystal retains at least about 80 percent of its initial activity after incubation for three hours in the presence of a concentration of Pronase™ protease that causes the soluble uncrosslinked form of the enzyme that is crystallized to form said enzyme crystal that is crosslinked to lose at least about 80 percent of its initial activity under the same conditions. Reaction mixtures useful for carrying out the foregoing method and product mixtures produced by the foregoing method are also disclosed.
A method for the enzymatic production of a product in a liquid or supercritical carbon dioxide fluid, said method comprising:
A mixture useful for the enzymatic production of a product in carbon dioxide fluid, said mixture comprising:
A mixture produced from the catalytic action of an enzyme on a substrate, said mixture comprising:
A method for the enzymatic production of a product in a liquid or supercritical carbon dioxide fluid, said method comprising:
A mixture useful for the enzymatic production of a product in carbon dioxide fluid, said mixture comprising:
A mixture produced from the catalytic action of an enzyme on a reaction substrate, said mixture comprising:
Dependencies are inferred from the claim text.