Metallocene-phosphoramidite conjugates, process for their preparation and their use
AssigneeDeutsches Krebsforschungszentrum Stiftung des Offentlichen Rechts
InventorsManfred Wiessler, Dagmar Schütte
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Abstract
The invention concerns metallocene-phosphoramidite conjugates comprising one or a plurality of metallocenes and one or a plurality of phosphoramidites. The invention further concerns a process for preparing the metallocene-phosphoramidite conjugates and their use.
Claims14
A process for preparing a metallocene-phosphoramidite conjugate, comprising:
- a) reacting a metallocene with a dicarboxylic acid anhydride or chloride to generate a metallocenoylalkyl carboxylic acid (I);
- b) reducing the compound obtained in step a) to generate an alcohol;
- and
- c) reacting the alcohol obtained in step b) with a phosphoramidite derivative to generate said metallocene-phosphoramidite conjugate.
A method for electron-microscopically detectable labeling of an isolated nucleic acid, comprising labeling said nucleic acid with a metallocene-phosphoramidite conjugate, wherein said metallocene-phosphoramidite conjugate comprises one or a plurality of metallocene units and one or a plurality of phosphoramidite units.
A method for cutting DNA, comprising exposing said DNA to metallocene-labeled oligonucleotides.
Dependencies are inferred from the claim text.