Back to results
US 6,211,349 B1Granted
Protonated/acidified nucleic acids and methods of use
AssigneeOligos Etc. Inc.
InventorsRoderic M. K. Dale, Amy Arrow, Steven L. Gatton, Terry Thompson
Life of this patent
Estimating the term…
Abstract
The novel discovery that protonated nucleic acids inhibit bacterial growth is reported and methods for the preparation and therapeutic use of nuclease resistant and acid resistant protonated/acidified nucleic acids for administration to animals including man, to treat or prevent a bacterial infection are described.
Claims19
A modified nucleic acid polymer comprising:
- a substitution at a residue of a ribose group, said substitution distinguishing said nucleic acid from naturally occurring RNA or DNA;
- and
- a blocking chemical modification at the 3′ terminus of the nucleic acid;
- wherein the nucleic acid is protonated to an extent that the nucleic acid exhibits a pH in a range of about 0 to about 5 at a concentration of 1 mg/ml in water, and the nucleic acid exhibits a pH stability of at least one hour at a pH of about 1 to about 5 at 37° C., and still further wherein the nucleic acid exhibits antibacterial activity.
1 more element
A modified nucleic acid comprising:
- a nucleotide monomer, said monomer comprising a blocking chemical modification at the 5′ position of the monomer;
- and a blocking chemical modification at the 3′ position of the monomer;
- and
- one or more exogenous protons introduced to reactive sites on said monomer,
- wherein the nucleic acid exhibits a pH stability of at least one hour in pH of 0.01 to 5, and wherein the nucleic acid exhibits antibacterial activity.
2 more elements
A composition comprising:
- a nucleic acid comprising a substitution at a residue of a ribose group, a blocking chemical modification at the 3′ terminus of the nucleic acid, wherein the nucleic acid is protonated to an extent that the nucleic acid exhibits a pH in a range of about 0 to about 5 at a concentration of 1 mg/ml in water;
- and
- an excipient;
- wherein the composition exhibits antibacterial activity.
1 more element
A composition comprising:
- a nucleotide monomer comprising a blocking chemical modification at the 5′ position of the monomer;
- a blocking chemical modification at the 3′ position of the monomer;
- and
- one or more exogenous protons introduced to reactive sites on said monomer,
- wherein the nucleic acid exhibits a pH stability of at least one hour in pH of 0.01 to 5, and wherein the nucleic acid exhibits antibacterial activity.
2 more elements
Dependencies are inferred from the claim text.