US 6,211,343 B1 · Lactoferrin receptor protein

Abstract

An isolated and purified lactoferrin receptor protein is isolated and purified from bacterial pathogens, including Moraxella and Neisseria, and has a molecular weight of between about 70,000 and about 90,000, as determined by SDS-PAGE. Such lactoferrin receptor protein may be provided in combination with a lactoferrin receptor protein from the bacterial pathogen of a molecular weight of about 100,000 to about 105,000 daltons. The lactoferrin receptor protein may be produced by providing a solubilized membrane preparation from the bacterial pathogen containing lactoferrin receptor proteins, non-lactoferrin receptor proteins and other contaminants, complexing the lactoferrin receptor proteins with lactoferrin and purifying the resulting complexes substantially free from the non-lactoferrin receptor proteins and the other contaminants, and separating the novel lactoferrin receptor protein from the complexes. The lactoferrin receptor protein is useful in diagnostic applications and immunogenic compositions, particularly for in vivo administration to a host to confer protection against disease caused by a bacterial pathogen that produces the lactoferrin receptor protein or produces a protein capable of inducing antibodies in a host specifically reactive with the lactoferrin receptor protein.

Claims6

Dependent claims

A method of producing a lactoferrin receptor protein from a bacterial pathogen selected from the group consisting of Neisseria meningitdis, Neisseria gonorrhoeae, Moraxella catarrhalis, Moraxella bovis and Moraxella lacunata, comprising the steps of:

(a) providing a solubilized membrane preparation from said bacterial pathogen containing lactoferrin receptor proteins, transferrin receptor proteins and other contaminants, said lactoferrin receptor proteins being a first lactoferrin receptor protein having a molecular weight of 100,000 to 105,000 daltons, as determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), and a second lactoferrin receptor protein having a molecular weight of between 70,000 and 90,000 daltons, as determined by SDS-PAGE,
(b) complexing both said first and second lactoferrin receptor proteins with lactoferrin and purifying the resulting complexes free from said transferrin receptor proteins and said other contaminants to provide purified complexes, and
(c) separating from the purified complexes both said first and second lactoferrin receptor proteins in the form of a mixture.

A method of producing a lactoferrin receptor protein from a bacterial pathogen selected from the group consisting of Neisseria menngitidis, Neisseria aonorrhoeae, Moraxella catarrhalis, Moraxella bovis and Moraxella lacunata, which comprises the steps of:

(a) providing a membrane preparation from the bacterial pathogen,
(b) solubilizing the membrane preparation to form a solubilized membrane preparation containing a selected lactoferrin receptor protein, transferrin receptor protein and other contaminants, wherein said selected lactoferrin receptor protein has a molecular weight of 100,000 to 105,000 daltons, as determined by SDS-PAGE
(c) complexing said selected lactoferrin receptor protein with lactoferrin and complexing said transferrin receptor protein with transferrin and purifying the resulting lactoferrin- and transferrin-complexes free from each other and said other contaminants to provide a first purified lactoferrin complex and purified transferrin complex, thereby removing said selected lactoferrin receptor protein and said transferrin receptor proteins from said solubilized membrane preparation to provide a depleted membrane preparation,
(d) solubilizing said depleted membrane preparation to form a solubilized membrane preparation containing a second lactoferrin receptor protein having a molecular weight of between 70,000 and 90,000 daltons, as determined by SDS-PAGE,
(e) complexing said second lactoferrin receptor protein with lactoferrin and removing the complex so formed from said depleted membrane preparation to provide second lactoferrin complexes,
(f) separating from the first lactoferrin complexes said selected lactoferrin receptor protein, and
(g) separating from the second lactoferrin complexes said second lactoferrin receptor protein.

4 more elements

Dependencies are inferred from the claim text.

Lactoferrin receptor protein

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Lactoferrin receptor protein

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Abstract

Claims6