Fluorescent dyes (AIDA) for solid phase and solution phase screening

Compounds represented by formula (I)

• wherein
• one of the radicals R1 or R2 and one of the radicals R3 or R4 is hydrogen and the other is independently —COOH, —COOR7, —CONH2, —CONH(CH2),OH, wherein n=2-8, —CONR8R9, —CH2OH, —CH2NH2, —NO2, NR10OR11, NHCOR12, Cl, Br, F, —CF3, —N=C=O, N=C=S, —SO3H, —SO2NH(CH2)nNH2, (C1-C4) alkyl, (C1-C16)-alkyl substituted at the terminal carbon with —COOH, —COOR7, —CONH2, —CONR8R9, —CONH(CH2)nOH, wherein n=2-8, —CH2OH, —CH2NH2, —N=C=O, N=C=S, —SO3H, —SO2NH(CH2)nNH2, —CONH(CH2)nNH2 wherein n=2-8, and the NH2-group could also be substituted by (C1-C4) alkyl or a commonly used amino protecting group,
• and one of the radicals R5 or R6 is hydrogen and the other is hydrogen, halogen, —NO2, NR10OR11, NHCOR12, (C1-C4) alkyl, (C1-C16)-alkyl substituted at the terminal carbon with —COOH, —COOR7, —CONH2, —CONR8R9, —CONH(CH2)nOH, wherein n=2-8, —CH2OH, —CH2NH2 , —N=C=O, N=C=S, —SO3H, —SO2NH(CH2)nNH2, —CONH(CH2)nNH2 wherein n=2-8, and the NH2-group could also be substituted by (C1-C4) alkyl or a commonly used amino protecting group,
• with the proviso that only one of R1-R6 is nitro,
• R7 is a commonly used carboxyl protecting or carboxyl activating group,
• R8 or R9 is hydrogen and the other is lower alkyl (C1-C4), phenyl, benzyl, or R8 and R9 are part of a 5 or 6 membered ring,
• R10 and R11 are independently hydrogen or (C1-C4)alkyl, and
• R12 is (C1-C10)alkyl or phenyl, which both can be substituted by (C1-C4) alkyl, protected amino group or halogen.